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An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.
Pubmed ID: 17183662
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Fisher Scientific Isotemp 280A is a Vacuum Oven offers a temperature range from ambient up to 200C with internal dimensions of 11.125"D x 9.75"W x 9.75"H. Front-mounted power indicator and dial thermometer with top-mounted analog vacuum gauge. The 280A's hydraulic thermostat controls temperature. Large glass panel front door and stainless steel chamber.
View all literature mentionsCell line K-562 is a Cancer cell line with a species of origin Homo sapiens (Human)
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