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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
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    • RRID:WB-STRAIN:WBStrain00030691

http://www.wormbase.org/db/get?name=WBStrain00030691

Source Database: WormBase (WB)
Affected Genes: WBGene00002974(lev-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00002974(lev-1), WBGene00006789(unc-54)
Availability: available
Source References: PMID:10806111
Synonyms: lev-1(e211) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1034, CGC_PJ1034
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Resistant to 1 mM levamisole.|"Made_by: Szewczyk/Jacobson"

Proper citation: RRID:WB-STRAIN:WBStrain00030691 Copy   

    • RRID:WB-STRAIN:WBStrain00030698

http://www.wormbase.org/db/get?name=WBStrain00030698

Source Database: WormBase (WB)
Affected Genes: WBGene00000481(cha-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000481(cha-1), WBGene00006789(unc-54)
Availability: available
Source References: PMID:10806111
Synonyms: cha-1(p1182) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1046, CGC_PJ1046
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc lethal at 25C. Difficult to score <25C.

Proper citation: RRID:WB-STRAIN:WBStrain00030698 Copy   

    • RRID:WB-STRAIN:WBStrain00030696

http://www.wormbase.org/db/get?name=WBStrain00030696

Source Database: WormBase (WB)
Affected Genes: WBGene00006762(unc-25)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00006762(unc-25), WBGene00006789(unc-54)
Availability: unknown
Source References: PMID:10806111
Synonyms: unc-25(e156) III; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1044
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Recoils slightly when prodded; poor backward movement.

Proper citation: RRID:WB-STRAIN:WBStrain00030696 Copy   

    • RRID:WB-STRAIN:WBStrain00030714

http://www.wormbase.org/db/get?name=WBStrain00030714

Source Database: WormBase (WB)
Affected Genes: WBGene00001860(him-1)|WBGene00002335(let-60)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00001860(him-1), WBGene00002335(let-60), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: him-1(e879) I; let-60(ga89) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1100, CGC_PJ1100
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Males appear to mate poorly - not quantitatively measured but very poor success with matings.

Proper citation: RRID:WB-STRAIN:WBStrain00030714 Copy   

    • RRID:WB-STRAIN:WBStrain00030711

http://www.wormbase.org/db/get?name=WBStrain00030711

Source Database: WormBase (WB)
Affected Genes: WBGene00003030(lin-45)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003030(lin-45), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: lin-45(sy96) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1092, CGC_PJ1092
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V.

Proper citation: RRID:WB-STRAIN:WBStrain00030711 Copy   

    • RRID:WB-STRAIN:WBStrain00030719

http://www.wormbase.org/db/get?name=WBStrain00030719

Source Database: WormBase (WB)
Affected Genes: WBGene00000548(clr-1)|WBGene00003401(mpk-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000548(clr-1), WBGene00003401(mpk-1), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: clr-1(e1745) II; mpk-1(n2521) III; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1114, CGC_PJ1114
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. clr-1 is termperature-sensitive.

Proper citation: RRID:WB-STRAIN:WBStrain00030719 Copy   

    • RRID:WB-STRAIN:WBStrain00030718

http://www.wormbase.org/db/get?name=WBStrain00030718

Source Database: WormBase (WB)
Affected Genes: WBGene00000548(clr-1)|WBGene00003030(lin-45)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000548(clr-1), WBGene00003030(lin-45), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: clr-1(e1745) II; lin-45(sy96) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1110, CGC_PJ1110
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. sy96 appears to suppress the Clr phenotype of e1745. Lots of Bags and larval lethals.

Proper citation: RRID:WB-STRAIN:WBStrain00030718 Copy   

    • RRID:WB-STRAIN:WBStrain00030715

http://www.wormbase.org/db/get?name=WBStrain00030715

Source Database: WormBase (WB)
Affected Genes: WBGene00002335(let-60)|WBGene00003186(mek-2)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00002335(let-60), WBGene00003186(mek-2), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: mek-2(ku114) I; let-60(ga89) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1105, CGC_PJ1105
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Occasional bags and L1 lethality. ga89 is temperature sensitive. Maintain at 16C.

Proper citation: RRID:WB-STRAIN:WBStrain00030715 Copy   

    • RRID:WB-STRAIN:WBStrain00030725

http://www.wormbase.org/db/get?name=WBStrain00030725

Source Database: WormBase (WB)
Affected Genes: WBGene00003965(pdk-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003965(pdk-1), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: ccIs55 V; pdk-1(mg142) X.
Alternate IDs: WB-STRAIN:PJ1134, CGC_PJ1134
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. No visible phenotype (may be smallish??). Dominant suppressor of daf-c phenotype of age-1.

Proper citation: RRID:WB-STRAIN:WBStrain00030725 Copy   

    • RRID:WB-STRAIN:WBStrain00030723

http://www.wormbase.org/db/get?name=WBStrain00030723

Source Database: WormBase (WB)
Affected Genes: WBGene00000548(clr-1)|WBGene00004774(sem-5)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000548(clr-1), WBGene00004774(sem-5), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: clr-1(e1745) II; ccIs55 V; sem-5(n1779) X.
Alternate IDs: WB-STRAIN:PJ1126, CGC_PJ1126
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. clr-1 is temperature-sensitive. n1779 suppresses clr-1.

Proper citation: RRID:WB-STRAIN:WBStrain00030723 Copy   

    • RRID:WB-STRAIN:WBStrain00030702

http://www.wormbase.org/db/get?name=WBStrain00030702

Source Database: WormBase (WB)
Affected Genes: WBGene00002335(let-60)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00002335(let-60), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: let-60(ga89) IV; ccIs55 V.
Alternate IDs: WB-STRAIN:PJ1063, CGC_PJ1063
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and are almost sterile (brood size is 6).

Proper citation: RRID:WB-STRAIN:WBStrain00030702 Copy   

    • RRID:WB-STRAIN:WBStrain00030708

http://www.wormbase.org/db/get?name=WBStrain00030708

Source Database: WormBase (WB)
Affected Genes: WBGene00000548(clr-1)|WBGene00001184(egl-15)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000548(clr-1), WBGene00001184(egl-15), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: clr-1(e1745) II; ccIs55 V; egl-15(n1783) X.
Alternate IDs: WB-STRAIN:PJ1078, CGC_PJ1078
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. Non-Egl. Non-Scrawny. Class IV egl-15 mutation. Supresses the temperature-sensitive Clr phenotype.

Proper citation: RRID:WB-STRAIN:WBStrain00030708 Copy   

    • RRID:WB-STRAIN:WBStrain00030735

http://www.wormbase.org/db/get?name=WBStrain00030735

Source Database: WormBase (WB)
Affected Genes: WBGene00000898(daf-2)|WBGene00002992(lin-3)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000898(daf-2), WBGene00002992(lin-3), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: daf-2(m41) III; ccIs55 V; njEx38.
Alternate IDs: WB-STRAIN:PJ1166, CGC_PJ1166
Notes: ccIs55 [unc-54::lacZ + sup-7(st5)] V. njEx38 [unc-54p::daf-2(+) + goa-1p::GFP + rol-6(su1006)]. Maintain by picking Rollers. Arrest as dauers at 25C. Maintain at 15C.

Proper citation: RRID:WB-STRAIN:WBStrain00030735 Copy   

    • RRID:WB-STRAIN:WBStrain00030822

http://www.wormbase.org/db/get?name=WBStrain00030822

Source Database: WormBase (WB)
Affected Genes: WBGene00006789(unc-54)|WBGene00006829(unc-101)
Genomic Alteration: WBGene00006789(unc-54), WBGene00006829(unc-101)
Availability: available
Source References: PMID:8288128
Synonyms: unc-101(sy216)/hIn1 [unc-54(h1040)] I.
Alternate IDs: WB-STRAIN:PS968, CGC_PS968
Notes: Heterozygotes are WT and segregate embryonic lethals (sy216 homozygotes) and paralyzed Uncs (h1040 homozygotes). sy216 is a deletion of the unc-101 gene region. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.|"Mutagen: Trimethylpsoalen"

Proper citation: RRID:WB-STRAIN:WBStrain00030822 Copy   

    • RRID:WB-STRAIN:WBStrain00030966

http://www.wormbase.org/db/get?name=WBStrain00030966

Source Database: WormBase (WB)
Affected Genes: WBGene00004202(pry-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00004202(pry-1), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: pry-1(mu38)/hIn1 [unc-54(h1040)] I; syIs188.
Alternate IDs: WB-STRAIN:PS5551, CGC_PS5551
Notes: syIs188 [POPTOP + unc-119(+)]. Maintain by picking non-Uncs. syIs188 suppresses pry-1(mu38) Muv phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Proper citation: RRID:WB-STRAIN:WBStrain00030966 Copy   

    • RRID:WB-STRAIN:WBStrain00036087

http://www.wormbase.org/db/get?name=WBStrain00036087

Source Database: WormBase (WB)
Affected Genes: WBGene00001334(ero-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00001334(ero-1), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: ero-1(ok1287)/unc-54(e190) I.
Alternate IDs: WB-STRAIN:VC814, CGC_VC814
Notes: Mutagen:UV/TMP|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y105E8B.8. Homozygous lethal deletion chromosome balanced by unc-54(e190). Heterozygotes are WT, and segregate WT, paralyzed Unc (unc-54 homozygotes), and ok1287 homozygotes (probable early larval arrest). Strain is reasonably well balanced, but requires a bit of care. Presence of coily Uncs among progeny indicates recombination may have occurred; the nature of these animals is not known, but they are usually WT by PCR. Pick WT and check for correct segregation of progeny to maintain. Segregation ratio of WT:Unc should be 2:1 and not 3:1."

Proper citation: RRID:WB-STRAIN:WBStrain00036087 Copy   

    • RRID:WB-STRAIN:WBStrain00033362

http://www.wormbase.org/db/get?name=WBStrain00033362

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00018237(irg-7)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00018237(irg-7)
Availability: available
Source References: EMPTY
Synonyms: irg-7(ve536[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) X.
Alternate IDs: WB-STRAIN:RG3036, CGC_RG3036
Notes: Homozygous viable. Deletion of 5265 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: ccTTAGTTGTTGATACAGTAGACACCTCCA ; Right flanking sequence: GCTACAATTGTAACGTAGTCAACGCTAAGA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Homozygous viable. Deletion of 5265 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking Sequence: ccTTAGTTGTTGATACAGTAGACACCTCCA ; Right flanking sequence: GCTACAATTGTAACGTAGTCAACGCTAAGA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation."|"Made_by: RG KO Group"|"Superficially wild-type. CRISPR/Cas9 deletion of irg-7. Insertion site verified by PCR. myo-2p::GFP + NeoR cassette is still present but may be excised using LoxP sites. Left flanking sequence: ccTTAGTTGTTGATACAGTAGACACCTCCA Right flanking sequence: GCTACAATTGTAACGTAGTCAACGCTAAGA"

Proper citation: RRID:WB-STRAIN:WBStrain00033362 Copy   

    • RRID:WB-STRAIN:WBStrain00033365

http://www.wormbase.org/db/get?name=WBStrain00033365

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00009589(spe-36)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00009589(spe-36)
Availability: available
Source References: EMPTY
Synonyms: spe-36(ve539[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])/nT1[umnIs49] IV; +/nT1 V.
Alternate IDs: WB-STRAIN:RG3039, CGC_RG3039
Notes: F40F11.4. umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Homozygous Ste, lays only oocytes. Deletion of 2632 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, Ste GFP+ non-mKate2 (ve539 homozygotes), Vul non-GFP mKate2+ (nT1 homozygotes) and dead eggs. Maintain by picking wild-type GFP+ mKate2+. Left flanking Sequence: tcacaaaaactcacAAATAACTTTGTACCG ; Right flanking sequence: ACGCAAGAGCTATGAAGAACAGAATACATA. sgRNA #1: acAAATAACTTTGTACCGGG; sgRNA #2: CTTCATAGCTCTTGCGTCAC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: RG KO Group"|"Superficially wild-type. CRISPR/Cas9 deletion of F40F11.4. Insertion site verified by PCR. umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, ve539 homozygotes (Ste, lays only oocytes, GFP+ mKate-), Vul nT1 homozygotes (brighter mKate2+) and dead eggs. Maintain by picking wild-type GFP+ mKate2+. myo-2p::GFP + NeoR cassette is still present but may be excised using LoxP sites. Left flanking sequence: aaaactcacAAATAACTTTGTACCG Right flanking sequence: ACGCAAGAGCTATGAAGAACAGAAT"

Proper citation: RRID:WB-STRAIN:WBStrain00033365 Copy   

    • RRID:WB-STRAIN:WBStrain00033366

http://www.wormbase.org/db/get?name=WBStrain00033366

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: K06A9.3(ve541[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) X.
Alternate IDs: WB-STRAIN:RG3041, CGC_RG3041
Notes: Homozygous viable. Deletion of 469 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking Sequence: tgtcagaatcacaaaaaattatgttttttt ; Right flanking sequence: GGAGGGGCACATAGAATCAATCATGCGCGT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: RG KO Group"|"Superficially wild-type. CRISPR/Cas9 deletion of K06A9.3. Insertion site verified by PCR. myo-2p::GFP + NeoR cassette is still present but may be excised using LoxP sites. Left flanking sequence: gaatcacaaaaaattatgttttttt Right flanking sequence: GGAGGGGCACATAGAATCAATCATG"

Proper citation: RRID:WB-STRAIN:WBStrain00033366 Copy   

    • RRID:WB-STRAIN:WBStrain00033347

http://www.wormbase.org/db/get?name=WBStrain00033347

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00005050(sra-24)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00005050(sra-24), WBGene00006789(unc-54)
Availability: available
Source References: EMPTY
Synonyms: sra-24(ve520[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) II.
Alternate IDs: WB-STRAIN:RG3020, CGC_RG3020
Notes: Homozygous viable. Deletion of 1150 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: CCGAAGGTCTCACCAATGCATTGACCTCGA ; Right flanking sequence: CTTGGCGTTAATTATTTGAGAATATTCAAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Homozygous viable. Deletion of 1150 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking Sequence: CCGAAGGTCTCACCAATGCATTGACCTCGA ; Right flanking sequence: CTTGGCGTTAATTATTTGAGAATATTCAAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation."|"Made_by: RG KO Group"|"Superficially wild-type. CRISPR/Cas9 deletion of sra-39. Insertion site verified by PCR. myo-2p::GFP + NeoR cassette is still present but may be excised using LoxP sites. Left flanking sequence: TGAATTCGGTCTTGCCTCTTTTTTCCTAAC Right flanking sequence: TGAGGTACCTTGAAAAATAATAGCAAATAA"

Proper citation: RRID:WB-STRAIN:WBStrain00033347 Copy   

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