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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/btu1-1[K350M] (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1,T146G),HAc]; tax-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
Source References: PMID:12356864, PMID:16344310
Notes: pT146G HA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 (T146G) with HA tag under control of MTT1 promoter. #1 and #5 are two separate single cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01366 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr10-1[∆::NEO2] (WT; WT, ?)
Affected Genes: SFR10 (TTHERM_00392670)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Source References: PMID:23426847
Notes: Mates with UCB401
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03300 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (Circular permutation of HHO1 charge patch (see paper); pm-r, ?)
Affected Genes: HHO1 (TTHERM_00823720)
Source References: PMID:11972045
Notes: Somatic transformation of CU428 with HHO1 construct A-C
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02219 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-29; Basal body disorganization (TS)., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-29
Source References: PMID:21562224
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 K29A, E30A, D33A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03274 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Nulli 2 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00840 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb2[∆::neo2]/htb2[∆::neo2] (mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: Neo2 into HTB2 replacing coding Macronucleus: HTB2 coding replaced by neo2 but assorted to pm-s
Source References: PMID:19822522
Notes: This is the HTB2 germline KO homozygous heterokaryon. The 1.* series have different mating type from 10.* series.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02104 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,GFPc]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: C-terminal GFP- tag of HHT3, not sure if complete replacement
Source References: PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-GFP sequence. Neo2 in 5’ flanking of HHT3.
Mac transformant,GFP tagged HHT3, green nuclei.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01779 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi111[∆?::neo3] (twi11[∆?::neo3]/twi111[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Source References: PMID:16738316
Notes: ∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01811 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1 (GAL1/GAL1; gal-s, VI)
Source References: PMID:19036158
Notes: This is the strain used for mac genome sequencing project.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01539 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1,GFPc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal GFP tag into MTT1 locus, partial replacement
Source References: PMID:12356864, PMID:16344310
Notes: In mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region with GFP tag . Use the pTTMGG construct (GTU coding, GFP tag driven by MTT promoter) to rescue GTU KO heterokaryon.
Requires Cd to grow, Use .25 to 1 ug/ml. GFP not detected at protein level.
Proper citation: RRID:TSC_SD01417 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4/R,K7R,K10R,K13R,K21R]; pm-r, ?)
Affected Genes: HTA3 (TTHERM_00143660 )
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4R, K7R, K10R, K13R, K21R.
Source References: PMID:12665578, PMID:11430834
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTA3 K4R,K7R,K10R,K13R, K21R. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02070 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1::neo4/drh1::neo4. Neo in 5’end of gene,some coding remains (drh1[∆?::neo4 ] Neo in 5’end of gene,some coding remains; pm-r with Cd, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 Macronucleus: neo4
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ H7-4E.
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01944 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, R285D,K286D), HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation R285D, K286D and HA tag at c-teminus into the MTT1 locus, partial replacement.
Source References: PMID:12356864, PMID:16344310
Notes: rescued GTUKO 5x6 progeny having mutation R285D,K286D in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce. Not single celled yet. They were ~2 weeks old.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02516 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2] (mp-r, VII)
Affected Genes: HTB1 (TTHERM_00633360)
Genomic Alteration: Micronucleus: Neo2 into HTB1 replacing coding
Source References: PMID:19822522
Notes: This is the HTB1 germline KO homozygous heterokaryon. The 9.* series have different mating type from 11.* series.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02080 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A
Source References: PMID:18211962, PMID:18199688
Notes: IFT122 KO heterokaryon, this is the non- star side
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01791 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 3 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01031 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E433D,E435D]; pm-r)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU1 has only (E431, 432, 433, 435D) no WT
Source References: PMID:10831613
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,432,433,435D)
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02122 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HA-His-n]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA-His- tag of HHT3, not sure if complete replacement
Source References: PMID:16908532, PMID:9343391
Notes: HA-His-tagged hv2 transformant. Checked by western blot. HA-His-hv2 is expressed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02643 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K4Q] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
Source References: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-K4Q.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02091 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 3L (CHX1; pmr1-1; cy-s, pm-r, II)
Notes: Deletion on left arm of chromosome 3.
Proper citation: RRID:TSC_SD01040 Copy
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