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Species: Gallus gallus
Genetic Insert: Hen Egg lysozyme (Lyz) and H2Kb(TM)
Vector Backbone Description: Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.
Proper citation: RRID:Addgene_225211 Copy
Species: Gallus gallus
Genetic Insert: Duck Lysozyme and Chicken Ovalbumin(OVA)
Vector Backbone Description: Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.
Proper citation: RRID:Addgene_225573 Copy
Species: Gallus gallus
Genetic Insert: hen egg lysozyme
Vector Backbone Description: Backbone Marker:Pucci Lab; Vector Backbone:p7.NGFR.mCMV.hPGK.CD63GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.
Proper citation: RRID:Addgene_225572 Copy
Species: Gallus gallus
Genetic Insert: OVA-pdgfr
Vector Backbone Description: Backbone Marker:Naldini lab; Backbone Size:9213; Vector Backbone:p1074.NGFR.mCMV.hPGK.GFP.wpre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://ssrn.com/abstract=4912248 for preprint.
Proper citation: RRID:Addgene_225216 Copy
Species: Gallus gallus
Genetic Insert: LH2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9335341
Comments: full length LH-2B.
Proper citation: RRID:Addgene_16271 Copy
Species: Gallus gallus
Genetic Insert: NgCam
Vector Backbone Description: Vector Backbone:pBeta Actin-Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32959500
Comments: pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_162719 Copy
Species: Gallus gallus
Genetic Insert: Gallus gallus vinculin
Vector Backbone Description: Vector Backbone:pET15b/GVcl 1-1066 (Addgene #46171); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_162781 Copy
Species: Gallus gallus
Genetic Insert: ER81
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9814709
Comments: To make an antisense probe, cut with NotI and transcribe with T7.
Proper citation: RRID:Addgene_16274 Copy
Species: Gallus gallus
Genetic Insert: islet1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To make a sense probe, cut with XhoI and transcribe with T3. To make an antisense probe, cut with NotI transcribe with T7.
Proper citation: RRID:Addgene_16273 Copy
Species: Gallus gallus
Genetic Insert: LH-2A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16280 Copy
Species: Gallus gallus
Genetic Insert: enhanced-GFP-chicken ovalbumin tandem
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4731; Vector Backbone:eGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cloned by ananda Mookerjee and Thomas Weber
Proper citation: RRID:Addgene_163524 Copy
Species: Gallus gallus
Genetic Insert: PLA2G4A-C2 domain
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31050338
Proper citation: RRID:Addgene_170753 Copy
Species: Gallus gallus
Genetic Insert: FAK
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_21155 Copy
Species: Gallus gallus
Genetic Insert: FGF8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK (-); Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8548816
Comments: Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region.
For antisense probe linearize with EcoRI and use T7 Polymerase.
Proper citation: RRID:Addgene_22089 Copy
Species: Gallus gallus
Genetic Insert: Sprouty 1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Comments: The partial clone contains ~230bp coding region and 3' UTR sequences.
For antisense probe, linearize the plasmid with XbaI and use T3 Polymerase. For negative control sense probe linearize with XhoI and transcribe with T7 Polymerase.
Proper citation: RRID:Addgene_22095 Copy
Species: Gallus gallus
Genetic Insert: Sprouty 2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10498682
Comments: Partial chicken Sprouty2 cDNA clone was isolated from a chick embryonic brain cDNA library (Lambda Zap). The entire coding region and 3' UTR sequences are included.
For antisense probe, linearize the plasmid with EcoRI and use T7 Polymerase. The transcript should be approximately 1.4kb.
Proper citation: RRID:Addgene_22096 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Kedes Lab; Backbone Size:10200; Vector Backbone:pHBetaAPr-1-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3261634
Comments: The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector.
Proper citation: RRID:Addgene_22533 Copy
Species: Gallus gallus
Genetic Insert: Bromodomain containing 7
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_93
Proper citation: RRID:Addgene_19232 Copy
Species: Gallus gallus
Genetic Insert: Alpha 2 globin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_89
Proper citation: RRID:Addgene_19231 Copy
Species: Gallus gallus
Genetic Insert: Survivin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_94
Proper citation: RRID:Addgene_19233 Copy
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