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Species: E. coli
Genetic Insert: sgGal4
Vector Backbone Description: Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100549 Copy
Species: E. coli
Genetic Insert: AraC
Vector Backbone Description: Vector Backbone:pHG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34369028
Proper citation: RRID:Addgene_170114 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cas5
Vector Backbone Description: Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170090 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cse2
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170094 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1545810
Comments: The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and
LacZ::CS cut site was inserted into the HindIII site at the
upstream end of a promotorless LacZ gene on plasmid JH271.
The two LacZ sequences are in direct repeat orientation relative to each other.
The lambda sequences are of unknown origin
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21678 Copy
Species: E. Coli
Genetic Insert: ClpP-His6
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3426; Vector Backbone:pQE70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16237435
Comments: NGS results indicate S2P in ClpP
Proper citation: RRID:Addgene_22144 Copy
Species: E. Coli
Genetic Insert: K27 HMR
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15137760
Proper citation: RRID:Addgene_22865 Copy
Species: E. coli
Genetic Insert: Lvl0 5'-2xTetApt-mVenus-PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115962 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR SapI/BsaI Mut
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115947 Copy
Species: E. coli
Genetic Insert: polyphosphate kinase
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMMB206; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9143103
Proper citation: RRID:Addgene_18876 Copy
Species: E. coli
Genetic Insert: optimized maltose FRET sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18522753
Comments: W230A mutation, which we introduced to change the affinity.
Proper citation: RRID:Addgene_18853 Copy
Species: E. coli
Genetic Insert: arabinose transporter araE under control of Ptac
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMMB207; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11092865
Proper citation: RRID:Addgene_19001 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pFA6a-5FLAG-kanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19342 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P41nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19349 Copy
Species: E. coli
Genetic Insert: MetQ (N229A)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5652; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118268 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Comments: delta metNIQ E. coli strain BW2115 was a created in the Rees lab by modifying E. coli genomic DNA
Proper citation: RRID:Addgene_118258 Copy
Species: E. coli
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118257 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118259 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118256 Copy
Species: E. coli
Genetic Insert: 'tesA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20111002
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24625 Copy
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