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pIBA2-SpyTag-MBP
 
Resource Report
Resource Website 		RRID:Addgene_107422 Maltose-binding protein (MalE) E. coli Ampicillin PMID:30600549 Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7 Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:11:05 0
PVS10
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_104398 rpoA-rpoB-rpoC-6xHis and rpoZ E. coli Ampicillin PMID:25665556 Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and rpoZ ORFs under control of T7 promoter. Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:10:20 2
PIA900
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_104401 rpoA, B, C, and Z E. coli Ampicillin PMID:25665556 pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin 2026-09-01 09:10:20 3
pET28b – E. coli RecE residues 564-868
 
Resource Report
Resource Website 		RRID:Addgene_104534 RecE residues 564-866 (C-terminal nuclease domain) E. coli Kanamycin PMID:19446525 Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:10:23 0
pBad-HPII
 
Resource Report
Resource Website 		RRID:Addgene_105839 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:10:43 0
pBad-HPII TAG348
 
Resource Report
Resource Website 		RRID:Addgene_105844 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 348 2026-09-01 09:10:43 0
pBad-HPII TAG568
 
Resource Report
Resource Website 		RRID:Addgene_105845 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 568 2026-09-01 09:10:43 0
pBad-HPII TAG283
 
Resource Report
Resource Website 		RRID:Addgene_105843 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 283 2026-09-01 09:10:43 0
pSLQ1651-sgRNA(F+E)-sgGal4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_100549 sgGal4 E. coli Ampicillin PMID:28841410 Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 09:09:24 8
PRExpress
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_122486 LacZ E. coli Ampicillin PMID:28761084 Vector Backbone:pUASg; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:15:18 3
pcDNA3.1 mycBioID
 
Resource Report
Resource Website
50+ mentions 		RRID:Addgene_35700 BirA E. coli Ampicillin PMID:22412018 Backbone Marker:InVitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R118G (highly promiscuous form) 2026-09-01 09:53:26 93
pcDNA3.1 MCS-BirA(R118G)-HA
 
Resource Report
Resource Website
50+ mentions 		RRID:Addgene_36047 BirA(R118G)-HA E. coli Ampicillin PMID:22412018 Backbone Marker:Invitrogen; Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R118G (highly promiscuous form) 2026-09-01 09:53:28 64
pHL1561
 
Resource Report
Resource Website 		RRID:Addgene_37571 CsrA E. coli Kanamycin PMID:23878244 Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-09-01 09:53:45 0
pHL 1571
 
Resource Report
Resource Website 		RRID:Addgene_37760 ptet_ompC_gfpAaV E. coli Ampicillin PMID:22618873 Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin AaV degradation tag added to the 3'end 2026-09-01 09:53:47 0
pHL 1569
 
Resource Report
Resource Website 		RRID:Addgene_37759 ptet_ompC_gfpAaV E. coli Ampicillin PMID:22618873 Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin AaV degradation tag added to the 3'end 2026-09-01 09:53:47 0
pAT253
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_40943 GFP-LacI** E. coli Ampicillin PMID:21724830 The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253. Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization 2026-09-01 09:54:21 1
L5-LexAp65-L2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_41437 LexAp65 E. coli Kanamycin Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR P5-P2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:54:28 1
pCAG-AILR-LplA-ER
 
Resource Report
Resource Website 		RRID:Addgene_42574 LplA(AILR) with ER-retention sequence KDEL E. coli Ampicillin PMID:23181687 Backbone Size:4261; Vector Backbone:pCAG; Vector Types:; Bacterial Resistance:Ampicillin Compared to wild-type LplA: tryptophan 37 to alanine; threonine 57 to isoleucine; phenylalanine 147 to leucine; histidine 267 to arginine 2026-09-01 09:54:41 0
pBEST-p15A-PLlacO1-lacI-T500
 
Resource Report
Resource Website 		RRID:Addgene_45393 lac repressor e. coli Ampicillin PMID:20576148 Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:55:18 0
pET21-Streptavidin-Glutamate_Tag
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_46367 Streptavidin-Glutamate_Tag E. coli Ampicillin PMID:24056174 Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:55:29 14

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The Stimulating Peripheral Activity to Relieve Conditions (SPARC) effort is the result of National Institutes of Health’s (NIH) drive to map out the neural circuitry responsible for visceral control in higher vertebrates. The SPARC Anatomy Working Group (SAWG) is responsible for the integrity of anatomical knowledge in SPARC

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