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  • Organism:arabidopsis thaliana (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
 
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RRID:Addgene_71720 TRANSPORT INHIBITOR RESPONSE 1 Arabidopsis thaliana Ampicillin PMID:26552885 Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 Backbone Size:7373; Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin codon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated 2026-08-29 01:09:45 9
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
 
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RRID:Addgene_71719 auxin-responsive protein IAA17 Arabidopsis thaliana Ampicillin PMID:26552885 Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 Backbone Size:7373; Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin minimal degron sequence (71-114aa) with start codon 2026-08-29 01:09:45 4
pET28-At_PRORP1-His
 
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RRID:Addgene_67869 PRORP1 Arabidopsis thaliana Kanamycin PMID:22976545 The mitochondrial targeting sequence “MLRLTCFTPSFSRACCPLFAMMLKVPSVHLHHPRF“ of native precursor PRORP1 was replaced with the dipeptide “MG” Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28-b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Residues 36-572 (see comments) 2026-08-29 01:09:12 0
pMAL-PIAL2M-wt
 
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RRID:Addgene_67909 PIAL2 Arabidopsis thaliana Ampicillin PMID:25415977 Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pMAL-c2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E281-A496 2026-08-29 01:09:12 0
pABD823
 
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RRID:Addgene_67911 Cold temperature germinating 10 (CTG10) Arabidopsis thaliana Chloramphenicol PMID:29632208 Backbone Marker:Stratagene; Backbone Size:6494; Vector Backbone:pBD-GAL4 Cam; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:09:12 0
pET28-At_PRORP2-His
 
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RRID:Addgene_67870 PRORP2 Arabidopsis thaliana Kanamycin PMID:22976545 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28-b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:09:12 0
pUC119-gRNA
 
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RRID:Addgene_52255 guide RNA targeting AtPDS3 Arabidopsis thaliana Ampicillin PMID:23929339 gRNA target sequence GGACTTTTGCCAGCCATGGT This plasmid is used as a PCR template to assemble new desired guide RNA according to the strategy published in Figure S5 of our Nature Biotechnology paper. Backbone Size:3162; Vector Backbone:pUC119; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:48 6
pAC-VIOL
 
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RRID:Addgene_53087 zep Arabidopsis thaliana Chloramphenicol PMID:24506237 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10040; Vector Backbone:pAC-ZEAXipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol Lacks codons for the first 60 N-terminal amino acids that are thought to comprise the chloroplast transit sequence 2026-08-29 01:06:54 0
pAC-DELTA
 
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1+ mentions
RRID:Addgene_53273 lcyE Arabidopsis thaliana Chloramphenicol PMID:8798688 For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:56 1
pAtCHYb1Δ129N
 
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RRID:Addgene_53367 chyB1 Arabidopsis thaliana Ampicillin PMID:15659105 Backbone Marker:Invitrogen; Backbone Size:4414; Vector Backbone:pTrcHis A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Deleted codons for the first 129 amino acids 2026-08-29 01:06:57 0
AtUBQ10+pLB12
 
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RRID:Addgene_53449 UBQ10 promoter Arabidopsis thaliana Spectinomycin The vector pLB12 contains both an activator unit and a b-glucuronidase (GUS) reporter within a responder unit. Backbone Marker:Mark Curtis at ETH Zurich (Brand et al., Plant Physiology 2006); Vector Backbone:pLB12; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:06:57 0
pAtLCYbSK
 
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RRID:Addgene_53588 lcyB Arabidopsis thaliana Ampicillin PMID:17085635 Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:58 0
y2
 
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1+ mentions
RRID:Addgene_53586 lcyE Arabidopsis thaliana Ampicillin PMID:8837512 Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:06:58 1
pETM6-At4CL-CmCHS-CmCHI
 
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RRID:Addgene_73395 At4CL Arabidopsis thaliana Ampicillin PMID:26860871 Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5203; Vector Backbone:pETM6; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:10:02 0
pETM6-At4CL-PhCHS-CmCHI
 
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RRID:Addgene_73393 At4CL Arabidopsis thaliana Ampicillin PMID:26860871 Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5203; Vector Backbone:pETM6; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:10:02 0
pVP2A-PIF6
 
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1+ mentions
RRID:Addgene_73369 PIF6 Arabidopsis thaliana Ampicillin PMID:26618393 Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Backbone Size:7282; Vector Backbone:pRepCap; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:10:02 1
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
 
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RRID:Addgene_73404 At4CL Arabidopsis thaliana Ampicillin PMID:26860871 Identical to pETM6-At4CL-PhCHS-CmCHI above except that the CmCHI gene is expressed from the ePathOptimize C4 (mutant T7) promoter. Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5203; Vector Backbone:pETM6; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:10:02 1
pEG03
 
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RRID:Addgene_73841 PhyB(1-917) with C-term his tag Arabidopsis thaliana Ampicillin PMID:26618393 Backbone Marker:Dictybase (http://dictybase.org/) ; Backbone Size:6213; Vector Backbone:pNO001; Vector Types:Dictyostelium discoideum expression; Bacterial Resistance:Ampicillin 2026-08-29 01:10:06 0
JW616
 
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RRID:Addgene_64805 CUC2 Arabidopsis thaliana Ampicillin PMID:25448000 Vector Backbone:pGADT7; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:08:45 0
JW630
 
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RRID:Addgene_64807 TCP4 Arabidopsis thaliana Ampicillin PMID:25448000 Vector Backbone:pGADT7; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:08:45 0

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