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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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p15a-AsCas12f-apmR Resource Report Resource Website 1+ mentions |
RRID:Addgene_171610 | AsCas12f1 | Other | Apramycin | PMID:34475565 | Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-09-01 09:28:37 | 1 | ||
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pGM1192 Resource Report Resource Website 1+ mentions |
RRID:Addgene_115683 | mCherry, aac(3)IV, tsr | Synthetic | Apramycin | Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV | Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | mCherry codon optimized for Streptomyces | 2026-09-01 09:13:26 | 1 | |
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pGMGUS Resource Report Resource Website 1+ mentions |
RRID:Addgene_115678 | gusA, aac(3)IV, tsr | Streptomyces | Apramycin | Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV | Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin | 2026-09-01 09:13:26 | 2 | ||
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pSelAct-KO Resource Report Resource Website |
RRID:Addgene_174374 | Apramycin | PMID:34890250 | Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3). 1. First development of pSelAct for knockouts in Rhodococcus equi: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans: Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022 3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter: Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 | Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin | 2026-09-01 09:29:24 | 0 | |||
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pBAD_Ub11TCA Resource Report Resource Website |
RRID:Addgene_174517 | Ubiquitin | Homo sapiens | Apramycin | PMID:34083482 | Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | Recoded with TCA replacing codon 11 of wt ubiquitin | 2026-09-01 09:29:27 | 0 | |
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pMSR:MSMEG_0791:Dendra Resource Report Resource Website |
RRID:Addgene_174917 | MSMEG_0791 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0793:Dendra Resource Report Resource Website |
RRID:Addgene_174918 | MSMEG_0793 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0775:Dendra Resource Report Resource Website |
RRID:Addgene_174919 | MSMEG_0775 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0786:Dendra Resource Report Resource Website |
RRID:Addgene_174914 | MSMEG_0786 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:33 | 0 | |
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pMSR:MSMEG_0787:Dendra Resource Report Resource Website |
RRID:Addgene_174915 | MSMEG_0787 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0876:Dendra Resource Report Resource Website |
RRID:Addgene_174927 | MSMEG_0876 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0880:Dendra Resource Report Resource Website |
RRID:Addgene_174928 | MSMEG_0880 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0833:Dendra Resource Report Resource Website |
RRID:Addgene_174922 | MSMEG_0833 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0866:Dendra Resource Report Resource Website |
RRID:Addgene_174926 | MSMEG_0866 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0948:Dendra Resource Report Resource Website |
RRID:Addgene_174952 | MSMEG_0948 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0952:Dendra Resource Report Resource Website |
RRID:Addgene_174954 | MSMEG_0952 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0953:Dendra Resource Report Resource Website |
RRID:Addgene_174955 | MSMEG_0953 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0954:Dendra Resource Report Resource Website |
RRID:Addgene_174956 | MSMEG_0954 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0956:Dendra Resource Report Resource Website |
RRID:Addgene_174957 | MSMEG_0956 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 | |
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pMSR:MSMEG_0970:Dendra Resource Report Resource Website |
RRID:Addgene_174959 | MSMEG_0970 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-09-01 09:29:34 | 0 |
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