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Vector Backbone Description: Backbone Size:5450; Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:17117155
Proper citation: RRID:Addgene_110739 Copy
Species: Synthetic
Genetic Insert: Full-length tetO array (XbaI-NheI fragment of pLAU44)
Vector Backbone Description: Backbone Size:6803; Vector Backbone:pMF272; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:31821884
Comments: TetO arrays are unstable and dual selection with Ampicillin and Gentamycin are essential for the maintenance of the array. Confirm full-length plasmid by restriction digest and Sanger sequencing of any clones prior to use.
Some minor sequence discrepancies located in the TetO repeat array exist between Sanger and NGS based data. These are not of functional concern for use of the plasmid.
Proper citation: RRID:Addgene_131318 Copy
Species: Vibrio cholerae
Genetic Insert: tnsA
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186690 Copy
Species: Vibrio cholerae
Genetic Insert: tnsA
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186691 Copy
Species: Vibrio cholerae
Genetic Insert: tnsA
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
This vector is identical to pBFC0619, but it includes a dual restriction site (AsiSI+SbfI) flanking the Tn right end to reduce donor vector during ET-Seq.
Proper citation: RRID:Addgene_186694 Copy
Species: Scytonema hofmanni
Genetic Insert: tnsB
Vector Backbone Description: Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:34873292
Comments: Clone Tn barcodes using the 2xBsmBI cloning site.
Proper citation: RRID:Addgene_186693 Copy
Genetic Insert: dCas9, sgRNA expression cassette, sfGFP
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression, pir dependent origin of replication; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38126769
Proper citation: RRID:Addgene_208888 Copy
Species: Homo sapiens
Genetic Insert: Dipeptidyl peptidase 9
Vector Backbone Description: Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39552560
Proper citation: RRID:Addgene_254905 Copy
Species: Homo sapiens
Genetic Insert: Dipeptidyl peptidase 9
Vector Backbone Description: Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39552560
Proper citation: RRID:Addgene_254906 Copy
Species: Pseudomonas aeruginosa PA14
Genetic Insert: Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14
Vector Backbone Description: Backbone Marker:Herbert Schweizer (Addgene #63121); Backbone Size:4500; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40904105
Comments: Synthetic Type IF CRISPR-Cas system based on the sequence from Pseudomonas aeruginosa PA14 with a minimised version of the CRISPR 2 array containing a non-targeting spacer with BbsI restriction sites for cloning. Native promoter regions were cloned as the intergenic region between the CRISPR components and upstream genes. MluI, NsiI, NotI, NcoI, AscI restriction sites were added to make the system modular.
Proper citation: RRID:Addgene_246943 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt2A
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247009 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso1
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247005 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1A
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247006 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1K
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247007 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1A2A
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247012 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1K2K
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247013 Copy
Species: Homo sapiens
Genetic Insert: AEBP2iso2.mt1∆2∆
Vector Backbone Description: Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247014 Copy
Species: Homo sapiens
Genetic Insert: JARID2
Vector Backbone Description: Vector Backbone:pMB.Pos6; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:41168462
Proper citation: RRID:Addgene_247018 Copy
Species: Synthetic
Genetic Insert: mcherry
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201006 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201005 Copy
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