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Species: Other
Genetic Insert: T38M-P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49520 Copy
Species: Other
Genetic Insert: P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49519 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50365 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50366 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50369 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50368 Copy
Species: Pyrophorus plagiophthalamus
Genetic Insert: Click beetle red luciferase (optimized for Mycobacteria)
Vector Backbone Description: Backbone Marker:JD Cirillo; Backbone Size:4809; Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25265287
Proper citation: RRID:Addgene_60853 Copy
Species: Mycobacterium marinum
Genetic Insert: lpqZ
Vector Backbone Description: Backbone Size:5720; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:41384969
Proper citation: RRID:Addgene_240159 Copy
Species: Mycobacterium marinum
Genetic Insert: fecB (MMAR_1650)
Vector Backbone Description: Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:41384969
Proper citation: RRID:Addgene_240158 Copy
Species: Streptococcus pyogenes
Genetic Insert: S. pyogenes Cas9
Vector Backbone Description: Vector Backbone:2-micron/pUC; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned by homologous recombination in yeast following BglI/II digestion
Proper citation: RRID:Addgene_179079 Copy
Species: n/a
Genetic Insert: MG1655.ΔTAG.ΔTGA.ΔprfA.mprfB3.A37G-tRNA-Trp
Vector Backbone Description: Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39910296
Proper citation: RRID:Addgene_234622 Copy
Species: Plasmodiophora brassicae
Genetic Insert: PbGH3
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
Proper citation: RRID:Addgene_232335 Copy
Species: Mycobacterium tuberculosis
Genetic Insert: PPE18-ALFA
Vector Backbone Description: Backbone Marker:Bryson Lab; Backbone Size:6512; Vector Backbone:pBB115; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Proper citation: RRID:Addgene_231247 Copy
Species: Mycobacterium tuberculosis
Genetic Insert: PPE18-HA
Vector Backbone Description: Backbone Size:6512; Vector Backbone:pBB115; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Proper citation: RRID:Addgene_231246 Copy
Species: Escherichia coli
Genetic Insert: Deficient in the P2 bacteriophage packaging cos site; rhamnose-inducible P4 delta (δ) and epsilon (ε) genes
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33317264
Comments: P2 bacteriophage lysogen derived from E. coli EMG C-5545 strain.
We designed a pair of primers to check the presence of P2 lysogen in this strain:
- P2 gpH C-ter gpG Fw: 5’ GCAGAGCACGAACAGTCACG 3’
- P2 gpH C-ter gpG Rv: 5’ TTATTGCGGCATTTCCGGCC 3’
These primers amplify the C-terminal region of the P2 gpH gene (tail fiber) and the complete gpG gene (tail fiber chaperone) (PCR fragment size: 2070 bp).
Proper citation: RRID:Addgene_209018 Copy
Vector Backbone Description: Backbone Size:9915; Vector Backbone:pBAC2015, pGM1190, and pUC19; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227580 Copy
Species: Synthetic
Genetic Insert: EBFP2-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184461 Copy
Species: Synthetic
Genetic Insert: TagRFP-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184449 Copy
Species: Synthetic
Genetic Insert: TagRFP-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184447 Copy
Species: Synthetic
Genetic Insert: iRFP702-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184455 Copy
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