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Genetic Insert: PGL3 luciferase siRNA
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs
See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case.
Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence.
Proper citation: RRID:Addgene_15099 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
See article and author's map for more information.
Proper citation: RRID:Addgene_15098 Copy
Species: Mus musculus
Genetic Insert: Kir3.2
Vector Backbone Description: Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32372643
Proper citation: RRID:Addgene_140745 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
Species: Homo sapiens
Genetic Insert: dn-cHSF1
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:5840; Vector Backbone:pLenti CMV/TO Zeo Dest (644-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:26502114
Proper citation: RRID:Addgene_134734 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Green lab; Backbone Size:3301; Vector Backbone:Z1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:42490567
Proper citation: RRID:Addgene_135046 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Green lab; Vector Backbone:Z1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:42490567
Proper citation: RRID:Addgene_135612 Copy
Vector Backbone Description: Backbone Size:1933; Vector Backbone:pMB1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_127639 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:1933; Vector Backbone:pMB1 zeocin; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_127638 Copy
Species: Mus musculus
Genetic Insert: GIRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30902995
Proper citation: RRID:Addgene_124277 Copy
Species: Mus musculus
Genetic Insert: GIRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:30902995
Proper citation: RRID:Addgene_124278 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063803.
Proper citation: RRID:Addgene_133437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063802.
Proper citation: RRID:Addgene_133436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063799.
Proper citation: RRID:Addgene_133433 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pGAPZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063805.
Proper citation: RRID:Addgene_133423 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Backbone Size:3100; Vector Backbone:pGAPZalpha; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063804.
Proper citation: RRID:Addgene_133422 Copy
Species: Mycobacterium tuberculosis H37Rv
Genetic Insert: egfp
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3147; Vector Backbone:pGAPZ_ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126717 Copy
Species: Komagataella phaffii
Genetic Insert: ald1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3147; Vector Backbone:pGAPZ_ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126711 Copy
Species: Komagataella phaffii
Genetic Insert: acs1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3147; Vector Backbone:pGAPZ_ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126712 Copy
Species: Mycobacterium tuberculosis H37Rv
Genetic Insert: egfp
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126715 Copy
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