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Species: Synthetic
Genetic Insert: mCherry, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Comments: Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV
Proper citation: RRID:Addgene_115683 Copy
Species: Streptomyces
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV
Proper citation: RRID:Addgene_115678 Copy
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110085 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110088 Copy
Vector Backbone Description: Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_78576 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Comments: Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Proper citation: RRID:Addgene_125688 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spdCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Proper citation: RRID:Addgene_125687 Copy
Species: Other
Genetic Insert: AsCas12f1
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:34475565
Proper citation: RRID:Addgene_171610 Copy
Species: E. coli
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:30191290
Proper citation: RRID:Addgene_69615 Copy
Species: E. coli
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:30191290
Proper citation: RRID:Addgene_69994 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61736 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61737 Copy
Species: artificially synthesized, codon optimized gene from Strep pyogenes
Genetic Insert: Scocas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Bacterial Genome Editing; Bacterial Resistance:Apramycin
Defining Citation: PMID:25739462
Comments: More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences.
Proper citation: RRID:Addgene_62552 Copy
Species: Streptomyces
Genetic Insert: Streptomyces codon optimized the FnCas12a mutant EP16
Vector Backbone Description: Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:32695773
Proper citation: RRID:Addgene_177875 Copy
Vector Backbone Description: Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3).
1. First development of pSelAct for knockouts in Rhodococcus equi:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans:
Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022
3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174374 Copy
Vector Backbone Description: Backbone Size:5613; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126044 Copy
Vector Backbone Description: Backbone Size:5681; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126595 Copy
Vector Backbone Description: Backbone Size:5220; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126596 Copy
Species: Synthetic
Genetic Insert: tcp830
Vector Backbone Description: Backbone Size:4460; Vector Backbone:pSET152; Vector Types:Streptomyce integration vector derived from phiC31 int/attP; Bacterial Resistance:Apramycin
Defining Citation: PMID:15917435
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_127088 Copy
Species: Synthetic
Genetic Insert: XimD and PcPT
Vector Backbone Description: Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:32786357
Proper citation: RRID:Addgene_169109 Copy
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