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Species: Synthetic
Genetic Insert: mRuby2_PUFa
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71902 Copy
Genetic Insert: 4xNLS-PUFw-2xNLS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71905 Copy
Species: Synthetic
Genetic Insert: 4xNLS-PUFc-2xNLS
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71906 Copy
Species: Arabidopsis thaliana
Genetic Insert: UBQ10 promoter
Vector Backbone Description: Backbone Marker:Mark Curtis at ETH Zurich (Brand et al., Plant Physiology 2006); Vector Backbone:pLB12; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Comments: The vector pLB12 contains both an activator unit and a b-glucuronidase (GUS) reporter within a responder unit.
Proper citation: RRID:Addgene_53449 Copy
Species: Synthetic
Genetic Insert: BFPKRAB_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73692 Copy
Genetic Insert: plant 3'UTRpolyadenylation signal/terminator, Tom H4
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Other, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Proper citation: RRID:Addgene_51834 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS3B (AT5g38410, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50260 Copy
Species: Other
Genetic Insert: signal peptide, NbCRT calreticulin (Nicotiana benthamiana)
Vector Backbone Description: Vector Backbone:PICH41258; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50307 Copy
Species: Other
Genetic Insert: promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50268 Copy
Species: Other
Genetic Insert: N terminal HA tag (6x Human influenza hemagglutinin)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50300 Copy
Species: Homo sapiens
Genetic Insert: ZNF451
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3000; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257861
Comments: Represents the ORFeome subset of the TFome
Proper citation: RRID:Addgene_88070 Copy
Species: Homo sapiens
Genetic Insert: HIPK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23852 Copy
Species: Homo sapiens
Genetic Insert: ITK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23881 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 46881; Backbone Size:6733; Vector Backbone:pDL276; Vector Types:Bacterial Expression, Other, E. coli/Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:1409970
Comments: This plasmid may contain unstable elements. Prolonged growth in E. coli selects for deletions, but not in E. faecalis. Test multiple clones upon to ensure the full plasmid is selected.
Proper citation: RRID:Addgene_46882 Copy
Species: Other
Genetic Insert: GFP-GUS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8000; Vector Backbone:pB7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28784835
Proper citation: RRID:Addgene_167122 Copy
Species: Other
Genetic Insert: wtCas9
Vector Backbone Description: Backbone Size:1718; Vector Backbone:pcdf1-b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33397704
Comments: Please visit https://doi.org/10.1101/2020.06.13.149682 for bioRxiv preprint.
Proper citation: RRID:Addgene_157913 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:SpecR-pBBR1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180748 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK085; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180749 Copy
Species: Synthetic
Genetic Insert: pRha
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_187489 Copy
Species: Synthetic
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_187501 Copy
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