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Species: Other
Genetic Insert: VenusYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR/Zeo; Vector Types:Gateway Cloning; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71265 Copy
Species: Other
Genetic Insert: Beta-glucuronidase
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR221. attP1-ccdB-cmR-attP2 sequences of pDONR221 were cloned into pGEMt-easy; Vector Backbone:221-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71263 Copy
Species: Other
Genetic Insert: Tq2CFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71268 Copy
Species: Other
Genetic Insert: 3 times VenusYFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71271 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71270 Copy
Species: Other
Genetic Insert: T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71273 Copy
Species: Other
Genetic Insert: dGFP
Vector Backbone Description: Backbone Marker:Patrick Salmon; Backbone Size:5000; Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732825
Comments: Bisei Ohkawara recloned the CHOP promoter for me after a mix-up with the original plasmids. I received the good plasmids from Japan in June 2014.
from:
Division of Neurogenetics,
Center for Neurological Diseases and Cancer,
Nagoya University Graduate School of Medicine
Institute for advanced research Nagoya University (IAR)
65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan.
Proper citation: RRID:Addgene_71299 Copy
Species: Other
Genetic Insert: NLS-2xMCP-2xTagRFP-T
Vector Backbone Description: Vector Backbone:pHsp83; Vector Types:Insect Expression, P element-mediated germline transformation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_71242 Copy
Species: Other
Genetic Insert: loxP-hyg-cat-loxP
Vector Backbone Description: Backbone Marker:Jeffery Murry - Eric Rubin Lab; Vector Backbone:pJM1; Vector Types:Bacterial Expression, Source of loxP-hyg-cat- loxP for PCR amplification.; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25779316
Comments: This vector contains a hyg-cat cassette, flanked by loxP sites, that can be used for the construction of recombineeng substrates for M. smegmatis or M. tuberculosis. It contains a 1 bp change (relative to starting plasmid pJM1) at position 2008 (G to C) that eliminate a potential stop codon that could interfere with expression of downstream functions following excision of the cassette from the chromosome by Cre recombinase.
The hygromycin resistance gene encodes M13I and I111T variants compared to the NCBI reference [ADL66925.1]. These variants should not interfere with function.
Proper citation: RRID:Addgene_71487 Copy
Species: Other
Genetic Insert: 2x-mCherry
Vector Backbone Description: Backbone Size:4821; Vector Backbone:pKAN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26694838
Proper citation: RRID:Addgene_72236 Copy
Species: Other
Genetic Insert: KlURA3
Vector Backbone Description: Vector Backbone:pFA6aKlURA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260239
Comments: The Resnik and Storici Labs recommend reading the following review articles for more information on this plasmid:
- Storici and Resnick Method Enzymol 2006 PMID: 16793410
- Stuckey et al., Meth. Mol. Biol. 2011 PMID: 21660695
- Stuckey and Storici Methods Enzymol 2013 PMID: 24182920
Proper citation: RRID:Addgene_72238 Copy
Species: Other
Genetic Insert: iCre-P2A-tdTomato-T2A-Puro
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6677; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72255 Copy
Species: Other
Genetic Insert: FLPe-P2A-copGFp-T2A-Puro
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6677; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72258 Copy
Species: Other
Genetic Insert: copGFP-T2A-iCre
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6677; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72256 Copy
Species: Other
Genetic Insert: copGFP-T2A-Puro
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:7133; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72263 Copy
Species: Other
Genetic Insert: mCherry-T2A-Puro
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6227; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_72264 Copy
Species: Other
Genetic Insert: mIFP T2A HO1 IRES EGFP
Vector Backbone Description: Vector Backbone:pLentiCMV dest#2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098020
Proper citation: RRID:Addgene_72443 Copy
Species: Other
Genetic Insert: mCherry-puromycin cassetter
Vector Backbone Description: Backbone Marker:Rudolf Jaenisch (Addgene plasmid # 22075); Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA donor; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23900226
Comments: Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette. ccdB gene is nonfunctional.
Proper citation: RRID:Addgene_72596 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26018130
Proper citation: RRID:Addgene_66589 Copy
Species: Other
Genetic Insert: blasticidin
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBS SK; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28421278
Proper citation: RRID:Addgene_66950 Copy
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