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Species: Escherichia coli
Genetic Insert: HpaB
Vector Backbone Description: Backbone Marker:Addgene Plasmid # 49795 ; Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26488898
Proper citation: RRID:Addgene_71260 Copy
Species: Other
Genetic Insert: VenusYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR/Zeo; Vector Types:Gateway Cloning; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71265 Copy
Species: Other
Genetic Insert: Beta-glucuronidase
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR221. attP1-ccdB-cmR-attP2 sequences of pDONR221 were cloned into pGEMt-easy; Vector Backbone:221-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71263 Copy
Species: Other
Genetic Insert: Tq2CFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71268 Copy
Species: Homo sapiens
Genetic Insert: Sequestosome-1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22777351
Comments: This plasmid contains a T375A mutation within the Sequestosome-1 translation compared to NP_003891.1. The depositor states that this mutation does not affect plasmid function.
Proper citation: RRID:Addgene_71305 Copy
Species: Homo sapiens
Genetic Insert: Sequestosome-1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22777351
Comments: This plasmid contains a T375A mutation within the Sequestosome-1 translation compared to NP_003891.1. The depositor states that this mutation should not affect plasmid function.
Proper citation: RRID:Addgene_71306 Copy
Species: Homo sapiens
Genetic Insert: H-RAS-V12
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pBABE-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21119005
Comments: The insert within this plasmid contains a E143K mutation compared to NP_005334.1. The depositor states that this mutation should not affect plasmid function.
The HRAS coding sequence may have been codon optimized and differs from the NCBI reference sequences for this gene. Primer design and applications involving the use of the HRAS coding sequence should be based off of the Addgene NGS Result for accuracy
Proper citation: RRID:Addgene_71304 Copy
Species: Synthetic
Genetic Insert: VQR Cas9 variant
Vector Backbone Description: Vector Backbone:pCFJ601; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26680661
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_71309 Copy
Species: Homo sapiens
Genetic Insert: DDX5/17 shRNA
Vector Backbone Description: Backbone Marker:GE - Dharmacon; Backbone Size:13362; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26209609
Proper citation: RRID:Addgene_71307 Copy
Species: Homo sapiens
Genetic Insert: GM-CSF promoter -627 to +28 fragment
Vector Backbone Description: Backbone Marker:Cockerill lab, Addgene plasmid 71248; Vector Backbone:pXPG; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10611344
Comments: Before being inserted into pXPG, the GM-CSF promoter has been through successive rounds of recloning which have added cloning sites to the ends.
JM109 are the cells used by the author for pXPG and its derivatives.
Proper citation: RRID:Addgene_71250 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Num1-PH
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15023338
Proper citation: RRID:Addgene_71374 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh1-PH
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15023338
Proper citation: RRID:Addgene_71372 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh2-PH
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15023338
Proper citation: RRID:Addgene_71373 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9804818
Proper citation: RRID:Addgene_71378 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9804818
Proper citation: RRID:Addgene_71379 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10983984
Proper citation: RRID:Addgene_71377 Copy
Species: Synthetic
Genetic Insert: 2PH-PLC delta (Human)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway multisite middle entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_71283 Copy
Species: Homo sapiens
Genetic Insert: STIMATE (1-240)
Vector Backbone Description: Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26322679
Proper citation: RRID:Addgene_71280 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR, Plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26193878
Comments: Please see the cloning protocol attachments for detailed descriptions on how to insert sgRNA sequences into this plasmid.
Proper citation: RRID:Addgene_71287 Copy
Vector Backbone Description: Backbone Marker:Thomas Kuhlman; Backbone Size:5282; Vector Backbone:pTKDP-dhfr; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26332675
Proper citation: RRID:Addgene_71323 Copy
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