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Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138247 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138246 Copy
Species: Streptococcus thermophilus
Genetic Insert: dcas9 (Streptococcus thermophilus #3)
Vector Backbone Description: Backbone Size:5431; Vector Backbone:pVCERC-5; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31937638
Proper citation: RRID:Addgene_133319 Copy
Species: Enterobacteria phage lambda
Genetic Insert: cI
Vector Backbone Description: Vector Backbone:pFG036; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32963323
Comments: This plasmid was made to repress the expression of the transposase (pL promoter) in pFG051.
This plasmid has to be in the bacterium to avoid transposition of pFG051's transposon.
It has to be in the donor strain for conjugation to avoid unwanted transposition in the donor.
A strain carrying this plasmid was used to build pFG051.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_137996 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Backbone Marker:Native to E. coli Nissle; Backbone Size:3173; Vector Backbone:pMUT2; Vector Types:Bacterial Expression, Synthetic Biology, For use with E. coli Nissle in the mouse gut; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33301298
Proper citation: RRID:Addgene_137923 Copy
Vector Backbone Description: Vector Backbone:pYPQ133A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138100 Copy
Vector Backbone Description: Vector Backbone:pYPQ133A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138102 Copy
Vector Backbone Description: Vector Backbone:pYPQ133A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138101 Copy
Vector Backbone Description: Vector Backbone:pYPQ134A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138105 Copy
Vector Backbone Description: Vector Backbone:pYPQ131A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138096 Copy
Vector Backbone Description: Vector Backbone:pYPQ132A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138099 Copy
Genetic Insert: KJBAC1 Strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:17406455
Comments: E. coli bacterial strain that constitutively expresses high levels of lac repressor. Genotype: F- lacIq delta hisB463 delta(gpt-proAB-arg-lac) XIII
zaj::Tn10. Use 12.5 ug/ml tetracycline for growth.
Proper citation: RRID:Addgene_13424 Copy
Vector Backbone Description: Vector Backbone:pYPQ131C; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31730252
Proper citation: RRID:Addgene_134347 Copy
Genetic Insert: linker-mScarlet-I for C-terminal fusion
Vector Backbone Description: Vector Backbone:pMT178; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31550057
Proper citation: RRID:Addgene_134455 Copy
Vector Backbone Description: Vector Backbone:pBBR1MCS-2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34333160
Comments: Plasmid parts were assembled using Golden gate method
Proper citation: RRID:Addgene_134883 Copy
Genetic Insert: sox3gRNAT2
Vector Backbone Description: Vector Backbone:pU6; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127778 Copy
Genetic Insert: sox3gRNAT1
Vector Backbone Description: Vector Backbone:pU6; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127777 Copy
Genetic Insert: F' Tra operon
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16141338
Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.
Proper citation: RRID:Addgene_12082 Copy
Species: Salmonella typhimurium ATCC14028 Nal resistant
Genetic Insert: adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
Vector Backbone Description: Backbone Marker:Guzman et al, J Bacteriol, 1995, 177(14):4121-30; Backbone Size:4919; Vector Backbone:pBAD30; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:15306016
Proper citation: RRID:Addgene_126650 Copy
Vector Backbone Description: Backbone Size:17653; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30240613
Comments: Selectable Marker: Tetracycline; 30 μg/mL in E.coli and 100 μg/mL in Pseudomonas aeruginosa
Proper citation: RRID:Addgene_113347 Copy
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