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Species: Synthetic
Genetic Insert: phGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mycobacterium tuberculosis expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18641659
Comments: The pH-GFP insert in this plasmid contains a F220L mutation compared to the wild-type pHluorin. This difference does not affect function of the pH-GFP.
Proper citation: RRID:Addgene_70045 Copy
Species: Synthetic
Genetic Insert: Codon Optimized Peredox-mCherry
Vector Backbone Description: Backbone Size:4563; Vector Backbone:pMV762; Vector Types:Bacterial Expression, Shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:27878107
Proper citation: RRID:Addgene_90217 Copy
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:5997; Vector Backbone:BB3_AMA_2.8_pUC-ORI_L_AC_hph; Vector Types:Bacterial Expression, A. niger; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90278 Copy
Species: A. niger
Genetic Insert: gRNA (pyrg2)
Vector Backbone Description: Backbone Size:5997; Vector Backbone:BB3_AMA_2.8_pUC-ORI_L_AC_hph; Vector Types:Bacterial Expression, A. niger; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90277 Copy
Vector Backbone Description: Backbone Size:5994; Vector Backbone:pAL5000, pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28857325
Proper citation: RRID:Addgene_84268 Copy
Species: Discosoma sp
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5994; Vector Backbone:pMyCA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28857325
Proper citation: RRID:Addgene_84272 Copy
Genetic Insert: sgRNA cloning site
Vector Backbone Description: Backbone Marker:Addgene 20318; Backbone Size:6608; Vector Backbone:pTE-10M-0X; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:27407107
Proper citation: RRID:Addgene_84380 Copy
Vector Backbone Description: Backbone Marker:Wilmanns lab; Backbone Size:6876; Vector Backbone:pMyC; Vector Types:Bacterial Expression, Bacterial expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33006405
Proper citation: RRID:Addgene_84688 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Sheng Yang lab; Backbone Size:3500; Vector Backbone:pAHYB; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24822243
Proper citation: RRID:Addgene_85074 Copy
Vector Backbone Description: Backbone Marker:Sheng Yang Lab; Backbone Size:3500; Vector Backbone:pGHYB; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24822243
Proper citation: RRID:Addgene_87447 Copy
Genetic Insert: hyg
Vector Backbone Description: Backbone Marker:Yale Coli Genetic Stock Center; Backbone Size:6000; Vector Backbone:pCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24398052
Proper citation: RRID:Addgene_87831 Copy
Vector Backbone Description: Vector Backbone:pCOND; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110093 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110087 Copy
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110084 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110090 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126601 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126600 Copy
Vector Backbone Description: Backbone Size:5903; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126606 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119884 Copy
Species: Synthetic
Genetic Insert: hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31672972
Comments: pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134316 Copy
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