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Species: Geminigera cryophila
Genetic Insert: Anion channelrhodopsin GcACR_457
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6217; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28256618
Proper citation: RRID:Addgene_103137 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-132-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.
Proper citation: RRID:Addgene_103219 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-223-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.
Proper citation: RRID:Addgene_103369 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-483-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC. Please contact [email protected] if you are interested in this plasmid prior to placing an order.
Proper citation: RRID:Addgene_103550 Copy
Species: Homo sapiens
Genetic Insert: CIBN-tagBFP-hTRF2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts)
Proper citation: RRID:Addgene_103803 Copy
Species: Other
Genetic Insert: NLS-TetR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Proper citation: RRID:Addgene_103838 Copy
Species: Other
Genetic Insert: EGFP-TetR-NLS-VP16
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: replaced CMV promoter by SV2 promoter
Proper citation: RRID:Addgene_103834 Copy
Species: Other
Genetic Insert: EGFP-LacI-NLS-VP16
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: replaced CMV promoter by SV2 promoter
Proper citation: RRID:Addgene_103836 Copy
Species: Other
Genetic Insert: MS2 coat protein (MCP)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4678; Vector Backbone:pTagRFP_C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: relevance of mutations unknown, but insert sequence is almost identical (except A88G, I30V) to another published MCP sequence (GenBank: JQ624676.1, Auslaender et al. 2012, Nature 487(7405): 123-127)
Proper citation: RRID:Addgene_103831 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-iRFP713
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: Switched iRFP713 (PCR on Addgene #31857) for mCherry in pCRY2PHR-mCherry-N1 (Addgene #26866) using AgeI & NotI (AgeI site destroyed)
Proper citation: RRID:Addgene_103818 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: Switched EYFP for mCherry in pCRY2PHR-mCherry-N1 (Addgene #26866)
Proper citation: RRID:Addgene_103817 Copy
Species: Homo sapiens
Genetic Insert: CIBN-hTRF2-tagRFP-T
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts)
Proper citation: RRID:Addgene_103812 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-iRFP713-VP16
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: aa412-482 of UL48 (transactivation domain); mCherry was cut out from the backbone (XhoI/NotI cuts)
Proper citation: RRID:Addgene_103823 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-mCherry-VP16
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: aa412-482 of UL48 (transactivation domain); mCherry was cut out from the backbone (XhoI/NotI cuts) and reinserted with the insert
Proper citation: RRID:Addgene_103821 Copy
Species: Klebsiella pneumoniae
Genetic Insert: hph
Vector Backbone Description: Backbone Marker:Juergens H et al. Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA expression plasmid; Backbone Size:10046; Vector Backbone:not applicable; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29438517
Proper citation: RRID:Addgene_103872 Copy
Species: synthetic
Genetic Insert: CAS9-MSA
Vector Backbone Description: Backbone Size:4072; Vector Backbone:PCS2+; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Comments: non
Proper citation: RRID:Addgene_103882 Copy
Species: Riemerella anatipestifer
Genetic Insert: RanCas13b
Vector Backbone Description: Backbone Size:9000; Vector Backbone:EF1a-lenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29070703
Comments: Benchling link: https://benchling.com/s/seq-hxOBIW6sDZE1o4DMz6lZ
Proper citation: RRID:Addgene_103855 Copy
Genetic Insert: U6-LwaCas13aDR-BbsI-BbsI-polyT
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29070703
Comments: Benchling link: https://benchling.com/s/seq-0SqKieU2CWyd3RRawuKp
Proper citation: RRID:Addgene_103851 Copy
Species: Porphyromonas gulae
Genetic Insert: PguCas13b
Vector Backbone Description: Backbone Size:9000; Vector Backbone:EF1a-lenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29070703
Comments: Benchling link: https://benchling.com/s/seq-GYuyzloHGlD8CNO4TCSy
Proper citation: RRID:Addgene_103861 Copy
Species: Prevotella sp. P5-125
Genetic Insert: PspCas13b
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29070703
Comments: Benchling link: https://benchling.com/s/seq-HASFia3255bkdC9iUtxu
Proper citation: RRID:Addgene_103869 Copy
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