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Species: Other
Genetic Insert: Rep2/Cap5
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104964 Copy
Species: Magnetococcus marinus
Genetic Insert: His-NavMs, Voltage gated sodium channel
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:6200; Vector Backbone:pTracer-CMV2, IRES GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28205548
Comments: Note: The GFP variant in this plasmid is missing the last five amino acids compared to the closest reference sequence (AAC53663.1). The depositor has confirmed that this does not impact plasmid function.
Proper citation: RRID:Addgene_100004 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5433; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17220275
Comments: pcDNA3.0-HA-HIF1α P402A/P564A/N803A was made from pcDNA3.0-HA-HIF1α P402A/P564A ( Addgene (Plasmid #18955) using the QuikChange site-directed mutagenesis kit (Stratagene)
Proper citation: RRID:Addgene_87261 Copy
Species: Homo sapiens
Genetic Insert: human DNA-PKcs
Vector Backbone Description: Backbone Size:4665; Vector Backbone:pcmv6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16314509
Proper citation: RRID:Addgene_83317 Copy
Vector Backbone Description: Backbone Size:6213; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_80901 Copy
Species: Homo sapiens
Genetic Insert: BAP1
Vector Backbone Description: Backbone Marker:Rao lab (Addgene plasmid# 17442); Backbone Size:8200; Vector Backbone:MSCV-IRES-Thy1.1 DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26095772
Proper citation: RRID:Addgene_81024 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Size:5213; Vector Backbone:pBabe; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: The osTIR1-9Myc was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
Proper citation: RRID:Addgene_80074 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20551166
Comments: H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector.
Proper citation: RRID:Addgene_26790 Copy
Species: Mus musculus
Genetic Insert: autophagy-related 3 (yeast)
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20723759
Proper citation: RRID:Addgene_26926 Copy
Species: Homo sapiens
Genetic Insert: eGFPhTERT
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBABEhygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12198499
Proper citation: RRID:Addgene_28169 Copy
Species: Mus musculus
Genetic Insert: mTERT
Vector Backbone Description: Backbone Marker:Bob Weinberg (Addgene Plasmid #1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21732358
Proper citation: RRID:Addgene_36413 Copy
Species: Homo sapiens
Genetic Insert: hDna2
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBabe hygro 3xFLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22570476
Comments: to get 3XFLAG hDna2 out cut with BAMH1 and SAL1
Proper citation: RRID:Addgene_31955 Copy
Vector Backbone Description: Backbone Size:5729; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is an empty vector. Your gene can be inserted via LIC cloning.
8-series vectors are induced with L-arabinose for tighter control of expression. Glucose can be added to the medium to further inhibit leaky expression. The plasmid can be expressed in any E. coli line that lacks proteases.
8HR adds a TEV-cleavable His6 and a strep tag to the N terminus of your protein. The dual affinity tags can help to purify difficult proteins.
To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers.
Forward - 5'TACTTCCAATCCAATGCA3'
Reverse - 5'TTATCCACTTCCAATGTTATTA3'
Linearize the plasmid with SspI and gel purify.
When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector.
Visit http://qb3.berkeley.edu/qb3/macrolab/ for more information on this vector can be found through
Proper citation: RRID:Addgene_37505 Copy
Species: Homo sapiens
Genetic Insert: KIBRA
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22614006
Proper citation: RRID:Addgene_40887 Copy
Species: Mus musculus
Genetic Insert: mMCL1(T144D)
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe IRES puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19433446
Proper citation: RRID:Addgene_25388 Copy
Species: Homo sapiens
Genetic Insert: c-myc
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabepuro3:hbER tam; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15367674
Comments: Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam).
Proper citation: RRID:Addgene_19128 Copy
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2194165
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.
Proper citation: RRID:Addgene_1765 Copy
Vector Backbone Description: Backbone Size:4888; Vector Backbone:pBABE-zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2194165
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
SspI site in Amp gene.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Proper citation: RRID:Addgene_1766 Copy
Species: Homo sapiens
Genetic Insert: YTHDC1
Vector Backbone Description: Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34375583
Proper citation: RRID:Addgene_177129 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18094039
Comments: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8.
Proper citation: RRID:Addgene_22405 Copy
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