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Species: Synthetic
Genetic Insert: C-terminal HA tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128439 Copy
Genetic Insert: C terminal VSV tag
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26181 Copy
Genetic Insert: C terminal HA tag
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for N-terminal tagging
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26183 Copy
Vector Backbone Description: Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:18064608
Comments: pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag.
Proper citation: RRID:Addgene_18671 Copy
Species: Vibrio fischeri
Genetic Insert: ECFP, EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6724; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
https://www.nature.com/articles/npre.2008.1550.2
Proper citation: RRID:Addgene_24509 Copy
Species: Aequorea victoria (Jellyfish)
Genetic Insert: Yn, Yc, ECFP, EYFP
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:7434; Vector Backbone:pVitro2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
Proper citation: RRID:Addgene_24510 Copy
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161893 Copy
Species: Synthetic
Genetic Insert: pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
Vector Backbone Description: Backbone Marker:Christopher Voigt's Lab; Vector Backbone:pTHSSd_1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:33850130
Proper citation: RRID:Addgene_161937 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a T to C nucleotide mutation in attP1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141014 Copy
Species: Synthetic
Genetic Insert: MBP-NLUC3F10H
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31316580
Proper citation: RRID:Addgene_141291 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137884 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137883 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137885 Copy
Vector Backbone Description: Vector Backbone:pK18; Vector Types:Bacterial Expression, Cre/Lox, Herpesvirus Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:10888635
Comments: The BAC vector is derived from the E.coli F plasmid. The BAC vector was cloned into the high copy vector pK18, to increase yields. The BAC vector is typically excised by PacI. The BAC vector contains genes for participation (sopA, B,C, a repE gene (required for replication) and an origin of replication (oriS). There is also a chloramphenicol resistance gene. The BAC vector carries a gpt gene, driven by the HSV tk promoter; the gpt gene is followed by the SV40 polyA signal sequence (ca 125 bp). The BAC vector is terminally flanked by loxP sites. The plasmid contains also a 1.5 kb EcoRI-fragment from the murine herpesvirus MHV68. The sequence of pK18 is available from Genbank ACCESSION M17626.
Proper citation: RRID:Addgene_85709 Copy
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:4762; Vector Backbone:pDONR 221; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:28017692
Comments: Please note that there are some seqeunce discrepancies between Addgene's quality control sequences and the depositor's genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_85752 Copy
Vector Backbone Description: Vector Backbone:pMDC32; Vector Types:Plant Expression, Gateway compatible attR1-attR2 destination vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:28211909
Comments: Note: Addgene's quality control sequencing finds a 1.2kb transposable element upstream of the Kanamycin resistance cassette. This inserted sequence does not affect plasmid function.
Proper citation: RRID:Addgene_86207 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4774; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP5) and PstI (attP3) / EcoRV (attP5) sites
Proper citation: RRID:Addgene_186350 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4768; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP4) and PstI (attP3) / EcoRV (attP4) sites
Proper citation: RRID:Addgene_186351 Copy
Vector Backbone Description: Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23056524
Comments: To enhance folding of the fusion proteins a flexible linker was introduced between the coding sequence of either fragment of YFP and the attR recombination site. For more information see: http://www.ibmcp.upv.es/FerrandoLabVectors/
Proper citation: RRID:Addgene_89487 Copy
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