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Species: Homo sapiens
Genetic Insert: Human immunoglobulin gamma 1 constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_52214 Copy
Vector Backbone Description: Vector Backbone:pCOND; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110093 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110087 Copy
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110084 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110090 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126601 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126600 Copy
Vector Backbone Description: Backbone Size:5903; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126606 Copy
Species: Synthetic
Genetic Insert: Fab_Theo-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119048 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119884 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Wasabi. The Wasabi ORF is present at bp# 39-749.
Proper citation: RRID:Addgene_30174 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Teal. The Teal ORF is present at bp# 103-813.
Proper citation: RRID:Addgene_30176 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Crimson. The Crimson ORF is present at bp# 104-781.
Proper citation: RRID:Addgene_30178 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with EBFP2. The EBFP2 ORF is present at bp# 104-823.
Proper citation: RRID:Addgene_30177 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Addgene Plasmid 30171; Backbone Size:0; Vector Backbone:pMSP12::dsRed2; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Comments: This plasmid was derived from pMSP12::dsRed2 by interupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#4947 when compared to the full sequence information provided by the depositing laboratory. This mismatch is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_30170 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5304; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
Proper citation: RRID:Addgene_31852 Copy
Species: Homo sapiens
Genetic Insert: PIK3C3 and PIK3R4
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6321; Vector Backbone:pVitro2; Vector Types:Mammalian Expression, bicistronic; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18957027
Proper citation: RRID:Addgene_24055 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24659 Copy
Species: Synthetic gene
Genetic Insert: Turbo
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24658 Copy
Species: Synthetis gene
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24657 Copy
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