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Species: Homo sapiens
Genetic Insert: colony stimulating factor 1
Vector Backbone Description: Backbone Size:6573; Vector Backbone:MSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2966924
Proper citation: RRID:Addgene_86795 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5433; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17220275
Comments: pcDNA3.0-HA-HIF1α P402A/P564A/N803A was made from pcDNA3.0-HA-HIF1α P402A/P564A ( Addgene (Plasmid #18955) using the QuikChange site-directed mutagenesis kit (Stratagene)
Proper citation: RRID:Addgene_87261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS511b
Vector Backbone Description: Vector Backbone:p426; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27899650
Proper citation: RRID:Addgene_87387 Copy
Species: Homo sapiens
Genetic Insert: BIG1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Vector Backbone:pcDNA4c; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15385626
Comments: It is tricky to amplify the plasmid. After transformation of the plasmid into E. coli, not only the transformants grow very slowly (usually, it takes more than 20 h for the transformants to form visible colonies on ampicillin‐containing plates and colony size is smaller than usual one), but also their viability is drastically decreased during storage at 4C. Therefore, we routinely use fresh transformants for plasmid preparation. The plasmid yield decreases with the progress of the day after transformation. It is not recommended to make a pre-culture (~ 2ml) from a single colony for large scale culture. We strongly recommend that after transformation and recovery culture (for 1hr at 37C), bring all transformants to large scale culture (~ 200 ml) directly containing ampicillin.
Proper citation: RRID:Addgene_79433 Copy
Genetic Insert: NA
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26829286
Comments: If LacI is added to the cell (e.g., in a plasmid), the expression of RFP will be somewhat repressed (unless if IPTG is added), as there is a lacO between the promoter and the gene's ATG. Attempts to remove this lacO were unsuccessful - we suspect the higher RFP expression might lead to cell death (in the cloning strain, E. coli DH5alpha).
Please note this strain has a higher %GC than E. coli, so PCRing (and sequencing) may be difficult.
Proper citation: RRID:Addgene_79642 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Size:5213; Vector Backbone:pBabe; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: The osTIR1-9Myc was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
Proper citation: RRID:Addgene_80074 Copy
Species: Homo sapiens
Genetic Insert: KDM3A
Vector Backbone Description: Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25488809
Proper citation: RRID:Addgene_82332 Copy
Species: Homo sapiens
Genetic Insert: Fv-Caspase 8 (C/A)-2A-GFP
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20308068
Proper citation: RRID:Addgene_82713 Copy
Species: Homo sapiens
Genetic Insert: ZNF451
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3000; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257861
Comments: Represents the ORFeome subset of the TFome
Proper citation: RRID:Addgene_88070 Copy
Species: Homo sapiens
Genetic Insert: human DNA-PKcs
Vector Backbone Description: Backbone Size:4665; Vector Backbone:pcmv6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16314509
Proper citation: RRID:Addgene_83317 Copy
Species: Homo sapiens
Genetic Insert: phlpp1
Vector Backbone Description: Backbone Size:5176; Vector Backbone:pBabe puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25201979
Proper citation: RRID:Addgene_84825 Copy
Genetic Insert: human IL-10
Vector Backbone Description: Vector Backbone:pHR SIN; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27693353
Proper citation: RRID:Addgene_85427 Copy
Species: Other
Genetic Insert: Csy4 hairpin
Vector Backbone Description: Backbone Size:5927; Vector Backbone:TR-GFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26354771
Proper citation: RRID:Addgene_80596 Copy
Species: Synthetic
Genetic Insert: NeoR
Vector Backbone Description: Backbone Size:2964; Vector Backbone:pBS-KS plus (BlueScribe KS Plus); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504702
Proper citation: RRID:Addgene_80759 Copy
Species: Synthetic
Genetic Insert: NeoR
Vector Backbone Description: Backbone Size:2964; Vector Backbone:pBS-KS plus (BlueScribe KS Plus); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504702
Proper citation: RRID:Addgene_80758 Copy
Vector Backbone Description: Backbone Size:6213; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_80901 Copy
Species: Homo sapiens
Genetic Insert: BAP1
Vector Backbone Description: Backbone Marker:Rao lab (Addgene plasmid# 17442); Backbone Size:8200; Vector Backbone:MSCV-IRES-Thy1.1 DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26095772
Proper citation: RRID:Addgene_81024 Copy
Species: Homo sapiens
Genetic Insert: human alpha 4 ectodomain
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:5600; Vector Backbone:pcDNA3.1 hygro(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29122968
Proper citation: RRID:Addgene_113406 Copy
Vector Backbone Description: Vector Backbone:p6000; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18944940
Proper citation: RRID:Addgene_113659 Copy
Species: Homo sapiens
Genetic Insert: Frataxin
Vector Backbone Description: Backbone Marker:pBABE-bleo/zeo is available at Addgene; Backbone Size:4888; Vector Backbone:pBABE bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035806
Proper citation: RRID:Addgene_11442 Copy
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