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Species: Synthetic
Genetic Insert: 2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and turboGFP expression vector for plants. Introns avoid expression in Agrobacteria. Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123190 Copy
Species: Synthetic
Genetic Insert: luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, Luciferase, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Renilla luciferase with an intron for transient and stable expression in plants. Codon optimized for plants.
Proper citation: RRID:Addgene_123181 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123221 Copy
Species: Agrobacterium tumefaciens
Genetic Insert: VirGN54D from pAD1289 (a gift from A. Das)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:12773; Vector Backbone:pAGM4723; Vector Types:Plant Expression, Level 2 acceptor vector for expression in plants ; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).
Proper citation: RRID:Addgene_123187 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123223 Copy
Species: P1 coliphage
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:5100; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31028261
Proper citation: RRID:Addgene_123133 Copy
Species: Mycobacterium smegmatis
Genetic Insert: Bxb1 integrase
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31028261
Proper citation: RRID:Addgene_123132 Copy
Species: Mus musculus
Genetic Insert: IkB alpha M
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8864120
Comments: IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment.
Proper citation: RRID:Addgene_12329 Copy
Species: Mus musculus
Genetic Insert: IkB alpha
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8864120
Proper citation: RRID:Addgene_12331 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pDEST-CMV-N-Tandem-mCherry-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123235 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pLenti-C-MYC-DDK Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30373810
Proper citation: RRID:Addgene_123299 Copy
Species: Homo sapiens
Genetic Insert: CSNK1A1
Vector Backbone Description: Vector Backbone:pLCE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29954751
Proper citation: RRID:Addgene_123320 Copy
Species: Homo sapiens
Genetic Insert: ArchT-BFP2
Vector Backbone Description: Backbone Marker:Michael Lin lab; Backbone Size:6105; Vector Backbone:pcDNA3.1/puro-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30638447
Proper citation: RRID:Addgene_123312 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based NE sensor GRAB_NE1m
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30922875
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/23/449546.full.pdf for BioRxiv preprint.
Proper citation: RRID:Addgene_123308 Copy
Species: Synthetic
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Size:9004; Vector Backbone:pRG01; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962622
Proper citation: RRID:Addgene_123363 Copy
Species: Drosophila melanogaster
Genetic Insert: U6:3-gRNA
Vector Backbone Description: Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite:
Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111
In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)).
Proper citation: RRID:Addgene_123366 Copy
Species: Mus musculus
Genetic Insert: GSDMD
Vector Backbone Description: Backbone Marker:Berkeley Structural Genomics Center; Backbone Size:6494; Vector Backbone:pDB-His-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30361383
Proper citation: RRID:Addgene_123365 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14742905
Proper citation: RRID:Addgene_12337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CKAR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123348 Copy
Species: Homo sapiens
Genetic Insert: NEP-X
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14742905
Comments: The sequence and map shown are for the wild-type Nep plasmid. The NepX plasmid contains an inactivating E585V mutation in the Nep gene.
Proper citation: RRID:Addgene_12340 Copy
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