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Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24664 Copy
Genetic Insert: pKW08-Lx-Int
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25015 Copy
Genetic Insert: pmyc1tetO-lacZ, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.
Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17971 Copy
Genetic Insert: tetR(B)r1.7
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.
Derivative of pMS2 with tetR r1.7 expressed by Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17973 Copy
Genetic Insert: pmyc1tetO-gfp, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.
pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+
Proper citation: RRID:Addgene_17975 Copy
Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20320 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
Species: A. niger
Genetic Insert: gRNA1 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118078 Copy
Vector Backbone Description: Backbone Marker:Matthias Steiger Martin Altvater; Backbone Size:2852; Vector Backbone:MST607; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118077 Copy
Species: A. niger
Genetic Insert: gRNA2 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118079 Copy
Species: A. niger
Genetic Insert: pTPI1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118075 Copy
Species: Synthetic
Genetic Insert: Fab_Theo-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119048 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119884 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117232 Copy
Species: M. smegmatis
Genetic Insert: gfp, hyg
Vector Backbone Description: Vector Backbone:pYS2; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24100088
Comments: Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117490 Copy
Species: Other
Genetic Insert: Htb2-GFP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_117669 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AB; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117827 Copy
Species: Other
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pMV261; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: The other inserted are "crRNA to ligD" and "hygromysin resistance gene". "crRNA to ligD" species is synthetic, promoter is Pj23119. Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104625 Copy
Vector Backbone Description: Vector Backbone:pCOND; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110093 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110087 Copy
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