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Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18094039
Comments: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8.
Proper citation: RRID:Addgene_22405 Copy
Species: Homo sapiens
Genetic Insert: OTUD4
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:0; Vector Backbone:pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22594 Copy
Species: Homo sapiens
Genetic Insert: eGFPhTERT
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBABEhygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12198499
Proper citation: RRID:Addgene_28169 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Size:8589; Vector Backbone:pLenti Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124521
Comments: Addgene QC NGS analysis returned BLAST results with significant sequence identity to Lamin A/C.
Proper citation: RRID:Addgene_182673 Copy
Species: Homo sapiens
Genetic Insert: c-myc
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabepuro3:hbER tam; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15367674
Comments: Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam).
Proper citation: RRID:Addgene_19128 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/
Proper citation: RRID:Addgene_47328 Copy
Species: Homo sapiens
Genetic Insert: KIBRA
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22614006
Proper citation: RRID:Addgene_40887 Copy
Species: Homo sapiens
Genetic Insert: KRAS*G12V
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23246410
Proper citation: RRID:Addgene_46746 Copy
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pWSK29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547065
Proper citation: RRID:Addgene_172972 Copy
Species: Drosophila melanogaster
Genetic Insert: DmLSm1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17923697
Proper citation: RRID:Addgene_145880 Copy
Species: E. coli Tn7
Genetic Insert: TnsC(A225V)
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pGD108; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9055068
Proper citation: RRID:Addgene_17257 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854235
Proper citation: RRID:Addgene_172604 Copy
Genetic Insert: TetON-P2A-TetOFF-T2A-bsd
Vector Backbone Description: Backbone Size:7821; Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31268602
Comments: The plasmid can be used in conjunction with pLenti-Tet (Addgene #138086) that has the ORF for the gene of interest of which expression can then be induced with Tetracycline or Doxycyline in Mammalian cells.
Proper citation: RRID:Addgene_138085 Copy
Vector Backbone Description: Vector Backbone:pH6HTN; Vector Types:Bacterial Expression, Other, Cell Free Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_175298 Copy
Genetic Insert: Adenovirus 5 genomic region 25043-35938
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Adenoviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35098167
Proper citation: RRID:Addgene_175747 Copy
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2194165
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.
Proper citation: RRID:Addgene_1765 Copy
Species: Mus musculus
Genetic Insert: Ribo-GCaMP8m
Vector Backbone Description: Backbone Size:2868; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_167574 Copy
Species: Synthetic
Genetic Insert: HygR gene
Vector Backbone Description: Backbone Size:2648; Vector Backbone:pUC19; Vector Types:Synthetic Biology, Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31518064
Proper citation: RRID:Addgene_168129 Copy
Species: Other
Genetic Insert: miRFP670
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32411820
Proper citation: RRID:Addgene_159435 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725302.1
Proper citation: RRID:Addgene_152030 Copy
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