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Species: Xanthamonas oryzae
Genetic Insert: repeat NK6
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31041 Copy
Species: Streptococcus thermophilus
Genetic Insert: dcas9 (Streptococcus thermophilus #3)
Vector Backbone Description: Backbone Size:5431; Vector Backbone:pVCERC-5; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31937638
Proper citation: RRID:Addgene_133319 Copy
Genetic Insert: KJBAC1 Strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:17406455
Comments: E. coli bacterial strain that constitutively expresses high levels of lac repressor. Genotype: F- lacIq delta hisB463 delta(gpt-proAB-arg-lac) XIII
zaj::Tn10. Use 12.5 ug/ml tetracycline for growth.
Proper citation: RRID:Addgene_13424 Copy
Genetic Insert: F' Tra operon
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16141338
Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.
Proper citation: RRID:Addgene_12082 Copy
Genetic Insert: I-SceI nuclease
Vector Backbone Description: Backbone Marker:Daniel Aubert; Backbone Size:7360; Vector Backbone:pDA17 (Broad host range plasmid recovered form Bordetella bronchiseptica); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18341581
Proper citation: RRID:Addgene_32061 Copy
Species: Homo sapiens
Genetic Insert: DUSP7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_27978 Copy
Species: Homo sapiens
Genetic Insert: DUSP7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_27976 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Comments: Y244C mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510
Proper citation: RRID:Addgene_27985 Copy
Vector Backbone Description: Backbone Size:8136; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11483365
Comments: A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3929. The depositing scientist says this deletion has no impact on the function of the plasmid.
Proper citation: RRID:Addgene_28037 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59399 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59398 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59775 Copy
Species: Other
Genetic Insert: tdh1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:4030087
Comments: Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1 cloned into the PstI site of pBR322.
Proper citation: RRID:Addgene_66158 Copy
Species: Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
Genetic Insert: B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
Vector Backbone Description: Vector Backbone:pSUP202pol4; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26035130
Proper citation: RRID:Addgene_67032 Copy
Species: Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
Genetic Insert: B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
Vector Backbone Description: Vector Backbone:pSUP202pol4; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26035130
Proper citation: RRID:Addgene_67030 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD8 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69280 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD5 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69277 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD2 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69274 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD7 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69279 Copy
Vector Backbone Description: Backbone Marker:(Cermak et al. 2011); Vector Backbone:pHD1 ; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26297141
Proper citation: RRID:Addgene_69287 Copy
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