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Species: Saccharomyces cerevisiae
Genetic Insert: VPS21
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT6
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18845 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GOS1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18848 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CHC1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin C-terminal hydrolase I
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15917625
Comments: Johnston SC, Riddle SM, Cohen RE, Hill CP. EMBO J. 1999 Jul 15;18(14):3877-87
Proper citation: RRID:Addgene_18895 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc28
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pYBS311; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Also called LexA-Cdc28
Proper citation: RRID:Addgene_18901 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase
Vector Backbone Description: Backbone Marker:Guzman et al.; Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_19041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP70 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBluescript SK+; Vector Types:carries FLP recombinase cDNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: erg3-mCherry
Vector Backbone Description: Backbone Size:7549; Vector Backbone:pESC/mCitrine (Addgene plasmid 117995); Vector Types:Plant Expression, Plant/bacteria binary vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30884945
Proper citation: RRID:Addgene_117993 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Brainbow 1.0 L
Vector Backbone Description: Vector Backbone:pT2-Ip2GI; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23757414
Proper citation: RRID:Addgene_118218 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPE1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For over expression experiments please use Escherichia coli BL21 or similar strains
Proper citation: RRID:Addgene_118343 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: inorganic diphosphatase IPP1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30632751
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/18/420570 for bioRxiv preprint.
Proper citation: RRID:Addgene_118978 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4
Vector Backbone Description: Backbone Size:2961; Vector Backbone:Bluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097066
Proper citation: RRID:Addgene_119013 Copy
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