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Species: Synthetic
Genetic Insert: mRuby2_PUFa
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Donor; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71902 Copy
Species: Synthetic
Genetic Insert: CTG REPEATS
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Addgene cannot verify or guarantee the presence of all 700 CTG repeats in this array
Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63087 Copy
Species: Homo sapiens
Genetic Insert: expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation).
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63089 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73169 Copy
Species: Synthetic
Genetic Insert: Clover_PUFc
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73689 Copy
Species: Synthetic
Genetic Insert: mRuby2_PUFa
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73690 Copy
Species: Homo sapiens
Genetic Insert: Malic Enzyme 1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23334421
Proper citation: RRID:Addgene_49163 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61887 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version does not contains a nuclear export signal. A related construct with the NES can be found at: http://www.addgene.org/47328/
Proper citation: RRID:Addgene_64945 Copy
Species: Synthetic
Genetic Insert: HygR gene
Vector Backbone Description: Backbone Size:2648; Vector Backbone:pUC19; Vector Types:Synthetic Biology, Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31518064
Proper citation: RRID:Addgene_168129 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854235
Proper citation: RRID:Addgene_172604 Copy
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pWSK29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547065
Proper citation: RRID:Addgene_172972 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21267423
Proper citation: RRID:Addgene_174006 Copy
Species: Other
Genetic Insert: CVB3
Vector Backbone Description: Backbone Marker:Invitrogen/Addgene #2092; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: There is an internal EcoRI site in the CVB3 sequence.
Proper citation: RRID:Addgene_158645 Copy
Species: Other
Genetic Insert: GFP-GUS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8000; Vector Backbone:pB7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28784835
Proper citation: RRID:Addgene_167122 Copy
Species: Mus musculus
Genetic Insert: Smad7
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe puro 3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10791980
Comments: See Author's map for more information.
Proper citation: RRID:Addgene_14836 Copy
Genetic Insert: Lineage tracing library
Vector Backbone Description: Backbone Marker:Allon Klein; Vector Backbone:pLARRY-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31974159
Proper citation: RRID:Addgene_140024 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Other, Gateway Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14193 Copy
Genetic Insert: TetON-P2A-TetOFF-T2A-bsd
Vector Backbone Description: Backbone Size:7821; Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31268602
Comments: The plasmid can be used in conjunction with pLenti-Tet (Addgene #138086) that has the ORF for the gene of interest of which expression can then be induced with Tetracycline or Doxycyline in Mammalian cells.
Proper citation: RRID:Addgene_138085 Copy
Species: E.coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16207908
Comments: Use gentamicin at 10 ug/mL.
Proper citation: RRID:Addgene_14467 Copy
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