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Species: Other
Genetic Insert: ccdB A-B
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54459 Copy
Species: Other
Genetic Insert: lacZ C-D
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54458 Copy
Species: Synthetic
Genetic Insert: hygromycin phosphotranferase (hptII) (pCAMBIA)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54463 Copy
Species: Synthetic
Genetic Insert: superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54461 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2500; Vector Backbone:pENTR-1A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
Proper citation: RRID:Addgene_55791 Copy
Species:
Genetic Insert: oriR6K
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Genbank Accession #AY884833
Proper citation: RRID:Addgene_64966 Copy
Species:
Genetic Insert: FRT-GmR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession #AY597270
Compared to the Genbank reference sequence AY597270, Addgene's sequencing results found a R98K mutation in the gentamycin cassette. The effect of this difference is unknown.
Proper citation: RRID:Addgene_64949 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Beckwith Lab; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_65098 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # DQ296496
Compared to the Genbank reference sequence DQ296496, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.
Proper citation: RRID:Addgene_65028 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # AY599228
Proper citation: RRID:Addgene_65022 Copy
Species:
Genetic Insert: ecfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # DQ493878
Proper citation: RRID:Addgene_65030 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC18T-mini-Tn7T-oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_63121 Copy
Species: Mus musculus
Genetic Insert: BICD2N (1-400)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pOmniBac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_64070 Copy
Species: Synthetic
Genetic Insert: BB0323-mCherry translational fusion under the control of the bb0826 promoter
Vector Backbone Description: Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Proper citation: RRID:Addgene_118244 Copy
Species: Synthetic
Genetic Insert: flagellin promoter driven iRFP
Vector Backbone Description: Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Proper citation: RRID:Addgene_118237 Copy
Species: Synthetic
Genetic Insert: flagellin promoter driven msfCFP
Vector Backbone Description: Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Proper citation: RRID:Addgene_118232 Copy
Species: Synthetic
Genetic Insert: flagellin promoter driven msfYFP
Vector Backbone Description: Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Proper citation: RRID:Addgene_118233 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Proper citation: RRID:Addgene_118225 Copy
Species: Synthetic
Genetic Insert: flagellin promoter driven mCherry
Vector Backbone Description: Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Proper citation: RRID:Addgene_118230 Copy
Species: jellyfish
Genetic Insert: gfpmut 3.1
Vector Backbone Description: Backbone Size:4768; Vector Backbone:pBBRMCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank accession number: JQ895027
pLMB509 contains GFPmut3.1 reporter gene downstream of the inducible promoter tauAp and a ribosomal binding site (RBS), followed by a six-His tag and a transcriptional stop codon. NdeI restriction sites flank the reporter gene, enabling the easy removal of gfpmut3.1 and subsequent replacement with the desired gene in frame.
This plasmid is compatible with BD In-Fusion cloning (Clontech) for the cloning of genes containing NdeI restriction sites
Proper citation: RRID:Addgene_40084 Copy
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