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Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26060277
Comments: Mariner himar1C9 transposase driven by lac promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Chloramphenicol resistance is contained on the plasmid backbone.
For further background, see:
Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017.
Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.
Proper citation: RRID:Addgene_91570 Copy
Species: Synthetic
Genetic Insert: CAMKIIA promoter and C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128461 Copy
Species: Synthetic
Genetic Insert: C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128440 Copy
Species: Synthetic
Genetic Insert: Ef1a promoter, N-terminal HA tag, and SV40 promoter
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128446 Copy
Species: Synthetic
Genetic Insert: C-terminal HA tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128439 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137884 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137883 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137885 Copy
Species: Synthetic
Genetic Insert: catP/sfGFP
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pGT-Ah7; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30643213
Comments: See supplemental tables of the referenced paper for more information on each plasmid.
Proper citation: RRID:Addgene_122574 Copy
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_124245 Copy
Species: Spinacia oleracea
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31085714
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
Proper citation: RRID:Addgene_126054 Copy
Species: Spinacia oleracea
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31085714
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
Proper citation: RRID:Addgene_126055 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM831
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84161 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM832-5p
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84163 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM838
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84166 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM837-5p
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84165 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM841a/b-5P
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84167 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM2939
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR.
Proper citation: RRID:Addgene_84114 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM390
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84117 Copy
Species: Arabidopsis thaliana
Genetic Insert: STTM393
Vector Backbone Description: Backbone Marker:Guiliang Tang (Addgene plasmid # 44182); Backbone Size:8608; Vector Backbone:pFGC5941-PacI; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23098881
Comments: The insert within this plasmid contains a 2x35S promoter, STTM, T35S and CmR. The STTM sequence in the plasmid contains discrepancies compared to the reference sequence. According to the depositing lab, these mutations should not affect plasmid function.
Proper citation: RRID:Addgene_84119 Copy
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