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Species: Other
Genetic Insert: TurboID-V5-eGFP
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135264 Copy
Species: Other
Genetic Insert: V5-eGFP
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135265 Copy
Species: Other
Genetic Insert: Flag-GFP
Vector Backbone Description: Backbone Size:9763; Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135275 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Bong-Kiun Kaang; Backbone Size:3771; Vector Backbone:CW3SL; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30397200
Proper citation: RRID:Addgene_118276 Copy
Species: Other
Genetic Insert: MvuTFIIB intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118397 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118399 Copy
Species: Other
Genetic Insert: CAS9p-TagRFP
Vector Backbone Description: Vector Backbone:pDONR-221z; Vector Types:CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32601420
Proper citation: RRID:Addgene_118386 Copy
Species: Other
Genetic Insert: CAS9p
Vector Backbone Description: Vector Backbone:pDONR-221z; Vector Types:CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32601420
Proper citation: RRID:Addgene_118385 Copy
Species: Other
Genetic Insert: MjaTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118393 Copy
Species: Other
Genetic Insert: MjaTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118395 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29868584
Proper citation: RRID:Addgene_118415 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118401 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118400 Copy
Species: Other
Genetic Insert: Tet-on 3G
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318302
Proper citation: RRID:Addgene_118592 Copy
Species: Other
Genetic Insert: EBFP2-ECFP
Vector Backbone Description: Vector Backbone:pQLinkHD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30339749
Proper citation: RRID:Addgene_118854 Copy
Species: Other
Genetic Insert: EBFP2
Vector Backbone Description: Vector Backbone:pQLinkHD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30339749
Proper citation: RRID:Addgene_118853 Copy
Species: Other
Genetic Insert: EBFP2-LSSmOrange
Vector Backbone Description: Vector Backbone:pQLinkHD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30339749
Proper citation: RRID:Addgene_118856 Copy
Species: Other
Genetic Insert: ECFP
Vector Backbone Description: Vector Backbone:pQLinkHD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30339749
Proper citation: RRID:Addgene_118857 Copy
Species: Other
Genetic Insert: NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pOET1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30925874
Proper citation: RRID:Addgene_118895 Copy
Species: Other
Genetic Insert: NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pOET1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30925874
Proper citation: RRID:Addgene_118898 Copy
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