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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAG415GAL-ccdB-EGFP
 
Resource Report
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1+ mentions
RRID:Addgene_14193 Chloramphenicol and Ampicillin PMID:17583893 Type of plasmid: CEN. This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid. Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Other, Gateway Destination; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-29 12:56:48 1
pRU1103
 
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1+ mentions
RRID:Addgene_14467 lacZ E.coli Gentamicin PMID:16207908 Use gentamicin at 10 ug/mL. Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin 2026-08-29 12:56:49 1
pLARRY-EGFP-bc(v1)
 
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1+ mentions
RRID:Addgene_140024 Lineage tracing library Ampicillin PMID:31974159 Backbone Marker:Allon Klein; Vector Backbone:pLARRY-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 12:56:35 6
mTert-pBabe-puro
 
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1+ mentions
RRID:Addgene_36413 mTERT Mus musculus Ampicillin PMID:21732358 Backbone Marker:Bob Weinberg (Addgene Plasmid #1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:04:32 5
pBAD Strep His6 TEV LIC cloning vector (8HR)
 
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1+ mentions
RRID:Addgene_37505 Ampicillin This plasmid is an empty vector. Your gene can be inserted via LIC cloning. 8-series vectors are induced with L-arabinose for tighter control of expression. Glucose can be added to the medium to further inhibit leaky expression. The plasmid can be expressed in any E. coli line that lacks proteases. 8HR adds a TEV-cleavable His6 and a strep tag to the N terminus of your protein. The dual affinity tags can help to purify difficult proteins. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. Visit http://qb3.berkeley.edu/qb3/macrolab/ for more information on this vector can be found through Backbone Size:5729; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:04:40 1
pBabe-HA-mMcl-1(T144D)
 
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1+ mentions
RRID:Addgene_25388 mMCL1(T144D) Mus musculus Ampicillin PMID:19433446 Backbone Size:5169; Vector Backbone:pBabe IRES puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin P142T T144D 2026-08-29 01:02:39 1
pBabe hygro 3xFLAG Dna2 wt
 
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1+ mentions
RRID:Addgene_31955 hDna2 Homo sapiens Ampicillin PMID:22570476 to get 3XFLAG hDna2 out cut with BAMH1 and SAL1 Backbone Size:5200; Vector Backbone:pBabe hygro 3xFLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Changed start ATG to AAA, 2026-08-29 01:03:54 3
pBABE-zeo (pBABE-bleo)
 
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10+ mentions
RRID:Addgene_1766 Ampicillin PMID:2194165 Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. SspI site in Amp gene. If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC. Backbone Size:4888; Vector Backbone:pBABE-zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:00:48 12
pLenti-Ef1a-mClover-YTHDC1-T2A-BSD-siRNA-Resistant
 
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1+ mentions
RRID:Addgene_177129 YTHDC1 Homo sapiens Ampicillin PMID:34375583 Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin siRNA resistant silent mutations 2026-08-29 01:00:53 1
pBABEpuro GFP-LC3
 
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50+ mentions
RRID:Addgene_22405 microtubule-associated protein 1 light chain 3 beta Rattus norvegicus Ampicillin PMID:18094039 LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8. Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:02:20 98
pBABEhygroHigheGFPhTERT
 
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1+ mentions
RRID:Addgene_28169 eGFPhTERT Homo sapiens Ampicillin PMID:12198499 Backbone Size:5500; Vector Backbone:pBABEhygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:03:27 5
pBabepuro-myc-ER
 
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10+ mentions
RRID:Addgene_19128 c-myc Homo sapiens Ampicillin PMID:15367674 Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam). Backbone Size:5169; Vector Backbone:pBabepuro3:hbER tam; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:01:41 16
pBabe TIR1-9myc
 
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10+ mentions
RRID:Addgene_47328 TRANSPORT INHIBITOR RESPONSE 1 Other Ampicillin PMID:23150568 Nishimura et al., 2009, Nature Methods Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/ Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:06:07 12
pBabepuro-KIBRA
 
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1+ mentions
RRID:Addgene_40887 KIBRA Homo sapiens Ampicillin PMID:22614006 Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:05:09 2
pBABE-Puro-KRas*G12V
 
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10+ mentions
RRID:Addgene_46746 KRAS*G12V Homo sapiens Ampicillin PMID:23246410 Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin carries the oncogenic G12V mutation and cDNA is encoded by a chimeric mixture of HRAS and KRAS codons that encode KRas protein 2026-08-29 01:06:03 14
pWSK29
 
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1+ mentions
RRID:Addgene_172972 Ampicillin PMID:16547065 Backbone Size:5400; Vector Backbone:pWSK29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:00:17 1
pRNA2-(A)128
 
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1+ mentions
RRID:Addgene_174006 eGFP Synthetic Kanamycin PMID:21267423 Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:00:24 1
pcDNA3.1-2xFLAG-2xSTREP (empty vector)
 
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1+ mentions
RRID:Addgene_172604 Ampicillin PMID:33854235 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:00:13 5
pTet-CTRL
 
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1+ mentions
RRID:Addgene_138085 TetON-P2A-TetOFF-T2A-bsd Ampicillin PMID:31268602 The plasmid can be used in conjunction with pLenti-Tet (Addgene #138086) that has the ORF for the gene of interest of which expression can then be induced with Tetracycline or Doxycyline in Mammalian cells. Backbone Size:7821; Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-29 12:56:14 1
pBABE-hygro
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_1765 Ampicillin PMID:2194165 Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC. Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning. Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:00:47 36

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