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Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTI site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN086 and pIN200, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255795 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUC site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN097 and pIN202, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255797 Copy
Species: Other
Genetic Insert: aadA gene flanked by flv3 upstream and downstream regions
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2986; Vector Backbone:pGEM®-T easy; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:36370921
Proper citation: RRID:Addgene_185533 Copy
Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431E T432E with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101836 Copy
Species: NA
Genetic Insert: sp/st resistance casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101846 Copy
Species: S. elongatus PCC7942
Genetic Insert: RpaA with its native promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101829 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli with point mutation E335Q
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100917 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160261 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160262 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160171 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160169 Copy
Species: Escherichia coli
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pSC101 rep-ts; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35411846
Proper citation: RRID:Addgene_183241 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:pTF505; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35690588
Proper citation: RRID:Addgene_187872 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:36129831
Proper citation: RRID:Addgene_190156 Copy
Species: Synthetic
Genetic Insert: RedNTF
Vector Backbone Description: Vector Backbone:pGreen; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:36550363
Proper citation: RRID:Addgene_198421 Copy
Species: Synthetic
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113637 Copy
Species: Mus musculus
Genetic Insert: Dishevelled-2
Vector Backbone Description: Backbone Marker:Novagen/EMD Biosciences; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35998232
Proper citation: RRID:Addgene_216387 Copy
Species: Synthetic
Genetic Insert: [PibpA-gfpASV]
Vector Backbone Description: Backbone Marker:Standard European Vector Architecture; Vector Backbone:pSEVA631; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:38676700
Proper citation: RRID:Addgene_221134 Copy
Species: Mus musculus
Genetic Insert: Dishevelled-2
Vector Backbone Description: Backbone Marker:Novagen/EMD Biosciences; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35998232
Proper citation: RRID:Addgene_216386 Copy
Species: Other
Genetic Insert: TALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI
Vector Backbone Description: Vector Backbone:pYPQ121; Vector Types:Plant Expression, Plastid base editing; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:38709858
Proper citation: RRID:Addgene_218172 Copy
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