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Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330.
Proper citation: RRID:Addgene_30175 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803.
Proper citation: RRID:Addgene_30179 Copy
Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:5087; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Proper citation: RRID:Addgene_37278 Copy
Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-rpsA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24663 Copy
Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24664 Copy
Genetic Insert: pKW08-Lx-Int
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25015 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
Proper citation: RRID:Addgene_31851 Copy
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:8221; Vector Backbone:pACE; Vector Types:Bacterial Expression, Escherichia coli-Mycobacterium shuttle plasmid; Bacterial Resistance:Hygromycin
Defining Citation: PMID:12754249
Comments: Plasmid can also be grown in mycobacteria.
Also see PMID 15342574 for additional reference.
Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional.
Proper citation: RRID:Addgene_31853 Copy
Genetic Insert: GFP (lacking ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
Proper citation: RRID:Addgene_31820 Copy
Genetic Insert: rpsL, FRT-hyg-FRT
Vector Backbone Description: Vector Backbone:pMN250; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:15563844
Comments: This is a suicide vector for gene deletions in mycobacteria; requires rpsL mutant (streptomycin resistant)
Proper citation: RRID:Addgene_32177 Copy
Genetic Insert: int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pMl603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference.
Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.
Proper citation: RRID:Addgene_32379 Copy
Genetic Insert: int Giles; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This is an improved Giles integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional
interference.
Proper citation: RRID:Addgene_32378 Copy
Genetic Insert: pJAZ38 ori
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11707328
Comments: This is a low copy number (one?) plasmid that co-replicates with pAL5000-derived plasmids in mycobacteria.
Proper citation: RRID:Addgene_32361 Copy
Species: Synthetic
Genetic Insert: mEos3.2_H-NSdbd
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168774 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168775 Copy
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pGIlesSHdI; Vector Types:Unspecified; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_170048 Copy
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pGilesSHdI; Vector Types:Unspecified; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_170047 Copy
Genetic Insert: pmyc1tetO-lacZ, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.
Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17971 Copy
Genetic Insert: tetR(B)r1.7
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.
Derivative of pMS2 with tetR r1.7 expressed by Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17973 Copy
Genetic Insert: pmyc1tetO-gfp, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.
pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+
Proper citation: RRID:Addgene_17975 Copy
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