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  • Bacterial Resistance:hygromycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGMEH-T38M-750-luxAB-DAS+4
 
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RRID:Addgene_49520 T38M-P750-luxAB-DAS+4 Other Hygromycin PMID:24191058 Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-29 01:06:25 0
pGMEH-0X750-luxAB-DAS+4
 
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RRID:Addgene_49519 P750-luxAB-DAS+4 Other Hygromycin PMID:24191058 Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-29 01:06:25 0
pCAS_gpyrG1
 
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1+ mentions
RRID:Addgene_90276 gRNA (pyrg1) A .niger Hygromycin PMID:28533066 Backbone Size:5997; Vector Backbone:BB3_AMA_2.8_pUC-ORI_L_AC_hph; Vector Types:Bacterial Expression, A. niger; Bacterial Resistance:Hygromycin 2026-08-29 01:12:43 1
pACBSR-hyg
 
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RRID:Addgene_87830 hyg Hygromycin PMID:24398052 Backbone Marker:N/A; Backbone Size:6300; Vector Backbone:pACBSR, which is based on pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin None 2026-08-29 01:12:25 0
pYUB28b-MCS-optimized
 
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RRID:Addgene_89179 Hygromycin PMID:21209917 Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details. This plasmid is identical to pYUB28b Plasmid #37277, except that a single change was introduced to the MCS (NcoI in the original plasmid is replaced by SphI in this version of the plasmid). Backbone Marker:Ghader Bashiri; Backbone Size:4920; Vector Backbone:pYUB28b Addgene #37277; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin 2026-08-29 01:12:32 0
pYUB28b-MBP
 
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RRID:Addgene_89178 none Hygromycin PMID:21209917 Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details. Note that the MBP tag in this plasmid is a surface entropy reduced version of the tag in order to help crytallisation down the line, and therefore has 6 alanine substitutions compared to the standard MBP sequence. Backbone Marker:Ghader Bashiri; Backbone Size:6040; Vector Backbone:pYUB28b Addgene #37277; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin 2026-08-29 01:12:32 0
pMyBADNT
 
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RRID:Addgene_84690 Hygromycin PMID:33006405 Backbone Marker:Wilmanns lab; Backbone Size:6902; Vector Backbone:pMyNT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-29 01:12:04 0
pVITRO-Fab_Quin-LUCID
 
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RRID:Addgene_119049 Fab_Quin-LUCID_part1 Synthetic Hygromycin PMID:28510347 Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:53:03 0
pVITRO-Fab_MTX-LUCID
 
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RRID:Addgene_119047 Fab_MTX-LUCID_part1 Synthetic Hygromycin PMID:28510347 Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:53:01 0
pDD132
 
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RRID:Addgene_119885 BbaJ23104_EYFP Hygromycin PMID:29366319 Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:53:08 0
pML2424
 
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RRID:Addgene_117497 Hygromycin PMID:22885758 Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication. Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin 2026-08-29 12:52:45 0
pYUB1062-GFP
 
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RRID:Addgene_117695 Hygromycin PMID:30319771 This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli. This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA. References: this paper has been accepted in RSC Advances: A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:52:50 0
pMEG_BB3_YL18H_AC
 
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RRID:Addgene_117828 Hygromycin PMID:30698703 Contains 34 bp GoldenMOCS-Yali Linker AB Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:52:49 0
pMST-1313
 
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RRID:Addgene_118076 pTEF1-cexA-tCYC1 A. niger Hygromycin PMID:30553933 Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:52:51 0
pBSV2H
 
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RRID:Addgene_118229 Hygromycin PMID:33257311 Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint. Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018. Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:52:53 0
pGWB2-CYCD3;1
 
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RRID:Addgene_140750 Cyclin D3;1 Arabidopsis thaliana Hygromycin PMID:31679162 Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin 2026-08-29 12:56:42 0
pTEC22
 
Resource Report
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1+ mentions
RRID:Addgene_30181 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:22840407 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522 Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin 2026-08-29 01:03:41 4
pTEC21
 
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RRID:Addgene_30180 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin 2026-08-29 01:03:41 0
pTEC27
 
Resource Report
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10+ mentions
RRID:Addgene_30182 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin 2026-08-29 01:03:42 32
pGFPHYG2
 
Resource Report
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1+ mentions
RRID:Addgene_30173 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:16714540 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin GFPmut3 allele has improved brightness. 2026-08-29 01:03:41 5

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