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Species: Other
Genetic Insert: T38M-P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49520 Copy
Species: Other
Genetic Insert: P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49519 Copy
Species: A .niger
Genetic Insert: gRNA (pyrg1)
Vector Backbone Description: Backbone Size:5997; Vector Backbone:BB3_AMA_2.8_pUC-ORI_L_AC_hph; Vector Types:Bacterial Expression, A. niger; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90276 Copy
Genetic Insert: hyg
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6300; Vector Backbone:pACBSR, which is based on pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24398052
Proper citation: RRID:Addgene_87830 Copy
Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:4920; Vector Backbone:pYUB28b Addgene #37277; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Comments: Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
This plasmid is identical to pYUB28b Plasmid #37277, except that a single change was introduced to the MCS (NcoI in the original plasmid is replaced by SphI in this version of the plasmid).
Proper citation: RRID:Addgene_89179 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:6040; Vector Backbone:pYUB28b Addgene #37277; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Comments: Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
Note that the MBP tag in this plasmid is a surface entropy reduced version of the tag in order to help crytallisation down the line, and therefore has 6 alanine substitutions compared to the standard MBP sequence.
Proper citation: RRID:Addgene_89178 Copy
Vector Backbone Description: Backbone Marker:Wilmanns lab; Backbone Size:6902; Vector Backbone:pMyNT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33006405
Proper citation: RRID:Addgene_84690 Copy
Species: Synthetic
Genetic Insert: Fab_Quin-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119049 Copy
Species: Synthetic
Genetic Insert: Fab_MTX-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119047 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119885 Copy
Vector Backbone Description: Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22885758
Comments: Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117497 Copy
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30319771
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.
This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam
Proper citation: RRID:Addgene_117695 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117828 Copy
Species: A. niger
Genetic Insert: pTEF1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118076 Copy
Vector Backbone Description: Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018.
Proper citation: RRID:Addgene_118229 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cyclin D3;1
Vector Backbone Description: Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31679162
Proper citation: RRID:Addgene_140750 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22840407
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522
Proper citation: RRID:Addgene_30181 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304.
Proper citation: RRID:Addgene_30180 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdTomato. The tdTomato ORF is present at bp# 102-1532.
Proper citation: RRID:Addgene_30182 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:16714540
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
Proper citation: RRID:Addgene_30173 Copy
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