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Species: E. coli
Genetic Insert: PrhaBAD-sfGFP
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3822; Vector Backbone:pJ404; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247275
Proper citation: RRID:Addgene_87767 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~2 minutes. See author's map.
Proper citation: RRID:Addgene_8818 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal >20 hours. See author's map.
Proper citation: RRID:Addgene_8816 Copy
Species: E. coli
Genetic Insert: Streptavidin-K121R-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757182
Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3)
In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)
Proper citation: RRID:Addgene_89880 Copy
Species: E. coli
Genetic Insert: Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757182
Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3)
In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)
Proper citation: RRID:Addgene_89881 Copy
Species: E. coli
Genetic Insert: LacI-11/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90059 Copy
Species: E. coli
Genetic Insert: LacI/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90058 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190243 Copy
Species: E. coli
Genetic Insert: Retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC])
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35896746
Comments: P35Q mutation in Cas2 insert does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187216 Copy
Species: E. coli
Genetic Insert: Retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC])
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35896746
Comments: P35Q mutation in Cas2 insert does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187212 Copy
Species: E. coli
Genetic Insert: Retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC])
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35896746
Comments: Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187210 Copy
Species: E. coli
Genetic Insert: Retron Eco1 Reverse Transcriptase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3900; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35896746
Comments: Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187218 Copy
Species: E. coli
Genetic Insert: Retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCA-TGC])
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35896746
Comments: P35Q mutation in Cas2 insert does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187217 Copy
Species: E. coli
Genetic Insert: Retron Eco1 Reverse Transcriptase
Vector Backbone Description: Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35896746
Comments: A192P mutation in mphR codon optimized does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187202 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170415 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170410 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170404 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170402 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.02.129247v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_162132 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.02.129247v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_162134 Copy
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