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Species: Homo sapiens
Genetic Insert: human APOAI gene
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12742997
Comments: Please note plasmid was difficult to sequence due to its size. Addgene NGS results contain several ambiguous bases. We advise running additional diagnostic tests before use.
The backbone was developed at BCM. The relevant reference is Proc. Natl. Acad Sci. USA 98: 13282-13287.
Proper citation: RRID:Addgene_124962 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125127 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125128 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125126 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125129 Copy
Species: Homo sapiens
Genetic Insert: SUN1_916
Vector Backbone Description: Backbone Marker:clonetech; Backbone Size:4700; Vector Backbone:ｐEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27858498
Proper citation: RRID:Addgene_125850 Copy
Species: Homo sapiens
Genetic Insert: PINK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8688; Vector Backbone:pLenti6/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15824318
Proper citation: RRID:Addgene_13318 Copy
Genetic Insert: 2x Streptococcus pyogenes sgRNA
Vector Backbone Description: Backbone Size:2739; Vector Backbone:pTAKN2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29403014
Proper citation: RRID:Addgene_108552 Copy
Species: Other
Genetic Insert: Rep2/Cap2
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104963 Copy
Species: Other
Genetic Insert: Rep2/Cap5
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104964 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-210-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.
Proper citation: RRID:Addgene_103346 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-210-5p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.
Proper citation: RRID:Addgene_103347 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-338-3p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.
Proper citation: RRID:Addgene_103450 Copy
https://www.addgene.org/kits/marillonnet-moclo/
Vector Backbone Description: Vector Types:Unspecified
Comments: pAGM1251 (Addgene #47995), pAGM1263 (Addgene #47985), pAGM1276 (Addgene #47986), pAGM1287 (Addgene #47996), pAGM1299 (Addgene #47988), pAGM1301 (Addgene #47989), pAGM1311 (Addgene #47983), pAGM4673 (Addgene #48014), pAGM4723 (Addgene #48015), pAGM8031 (Addgene #48037), pAGM8043 (Addgene #48038), pAGM8055 (Addgene #48039), pAGM8067 (Addgene #48040), pAGM8079 (Addgene #48041), pAGM8081 (Addgene #48042), pAGM8093 (Addgene #48043), pAGM9121 (Addgene #51833), pICH41233 (Addgene #47984), pICH41246 (Addgene #47992), pICH41258 (Addgene #47987), pICH41264 (Addgene #47993), pICH41276 (Addgene #47994), pICH41295 (Addgene #47997), pICH41308 (Addgene #47998), pICH41331 (Addgene #47999), pICH41722 (Addgene #48016), pICH41744 (Addgene #48017), pICH41766 (Addgene #48018), pICH41780 (Addgene #48019), pICH41800 (Addgene #48020), pICH41822 (Addgene #48021), pICH47732 (Addgene #48000), pICH47742 (Addgene #48001), pICH47751 (Addgene #48002), pICH47761 (Addgene #48003), pICH47772 (Addgene #48004), pICH47781 (Addgene #48005), pICH47791 (Addgene #48006), pICH47802 (Addgene #48007), pICH47811 (Addgene #48008), pICH47822 (Addgene #48009), pICH47831 (Addgene #48010), pICH47841 (Addgene #48011), pICH47852 (Addgene #48012), pICH47861 (Addgene #48013), pICH49244 (Addgene #48029), pICH49255 (Addgene #48023), pICH49266 (Addgene #48024), pICH49277 (Addgene #48025), pICH49283 (Addgene #48026), pICH49299 (Addgene #48027), pICH49300 (Addgene #48028), pICH50866 (Addgene #48022), pICH50872 (Addgene #48044), pICH50881 (Addgene #48045), pICH50892 (Addgene #48046), pICH50900 (Addgene #48047), pICH50914 (Addgene #48048), pICH50927 (Addgene #48049), pICH50932 (Addgene #48050), pICH53388 (Addgene #47990), pICH53399 (Addgene #47991), pICH54011 (Addgene #48065), pICH54022 (Addgene #48066), pICH54033 (Addgene #48067), pICH54044 (Addgene #48068), pICH54055 (Addgene #48069), pICH54066 (Addgene #48070), pICH54077 (Addgene #48071), pICH75322 (Addgene #48051), pICH75334 (Addgene #48052), pICH75344 (Addgene #48053), pICH75355 (Addgene #48054), pICH75366 (Addgene #48055), pICH75377 (Addgene #48056), pICH75388 (Addgene #48057), pICH79255 (Addgene #48058), pICH79264 (Addgene #48059), pICH79277 (Addgene #48060), pICH79289 (Addgene #48061), pICH79290 (Addgene #48062), pICH79300 (Addgene #48063), pICH79311 (Addgene #48064), pICH82094 (Addgene #48074), pICH82113 (Addgene #48073), pICH83955 (Addgene #48036), pICH83966 (Addgene #48030), pICH83977 (Addgene #48031), pICH83988 (Addgene #48032), pICH83999 (Addgene #48033), pICH84000 (Addgene #48034), pICH84011 (Addgene #48035), pICH86966 (Addgene #48075), pICH86988 (Addgene #48076), pICH89921 (Addgene #48072)
Proper citation: RRID:Addgene_1000000044 Copy
https://www.addgene.org/pooled-library/zhang-human-gecko-v2/
Vector Backbone Description: Vector Types:CRISPR, Lentiviral, Mammalian Expression
Comments: GeCKO v2 human library in lentiGuide-Puro A (Addgene #52959), GeCKO v2 human library in lentiGuide-Puro B (Addgene #52960), lentiCas9-Blast (Addgene #52962)
Proper citation: RRID:Addgene_1000000049 Copy
https://www.addgene.org/kits/yamamoto-multiplex-crispr/
Vector Backbone Description: Vector Types:CRISPR, Mammalian Expression
Comments: pX330A-1x2 (Addgene #58766), pX330A-1x3 (Addgene #58767), pX330A-1x4 (Addgene #58768), pX330A-1x5 (Addgene #58769), pX330A-1x6 (Addgene #58770), pX330A-1x7 (Addgene #58771), pX330A_D10A-1x2 (Addgene #58772), pX330A_D10A-1x3 (Addgene #58773), pX330A_D10A-1x4 (Addgene #58774), pX330A_D10A-1x5 (Addgene #58775), pX330A_D10A-1x6 (Addgene #58776), pX330A_D10A-1x7 (Addgene #58777), pX330S-2 (Addgene #58778), pX330S-3 (Addgene #58779), pX330S-4 (Addgene #58780), pX330S-5 (Addgene #58781), pX330S-6 (Addgene #58782), pX330S-7 (Addgene #58783)
Proper citation: RRID:Addgene_1000000055 Copy
https://www.addgene.org/kits/pcrisystem/
Vector Backbone Description: Vector Types:Bacterial Expression, Yeast Expression
Comments: pCri-11a (Addgene #61319), pCri-11b (Addgene #61333), pCri-12a (Addgene #61320), pCri-12b (Addgene #61334), pCri-13a (Addgene #61321), pCri-14a (Addgene #61322), pCri-14b (Addgene #61335), pCri-15a (Addgene #61323), pCri-15b (Addgene #61336), pCri-16a (Addgene #61324), pCri-17a (Addgene #61325), pCri-18a (Addgene #61326), pCri-1a (Addgene #60816), pCri-1a-Strep (Addgene #61337), pCri-1b (Addgene #61327), pCri-4a (Addgene #61314), pCri-4a-Strep (Addgene #61338), pCri-4b (Addgene #61328), pCri-6a (Addgene #61315), pCri-6b (Addgene #61329), pCri-7a (Addgene #61316), pCri-7a-Strep (Addgene #61339), pCri-7b (Addgene #61330), pCri-8a (Addgene #61317), pCri-8a-Strep (Addgene #61340), pCri-8b (Addgene #61331), pCri-9a (Addgene #61318), pCri-9a-Strep (Addgene #61341), pCri-9b (Addgene #61332)
Proper citation: RRID:Addgene_1000000058 Copy
https://www.addgene.org/kits/densmore-cidar-moclo/
Vector Backbone Description: Vector Types:Gateway Cloning, Synthetic Biology
Comments: B0015_DE (Addgene #66035), B0015_DF (Addgene #66036), B0015_DG (Addgene #66037), B0015_DH (Addgene #66038), B0032m_BC (Addgene #66020), B0033m_BC (Addgene #66021), B0034m_BC (Addgene #66022), BCD12_BC (Addgene #66023), BCD2_BC (Addgene #66024), BCD8_BC (Addgene #66025), C0012m (lacI)_CD (Addgene #66026), C0040 (tetR)_CD (Addgene #66027), C0062 (luxR)_CD (Addgene #66028), C0080 (araC)_CD (Addgene #66029), cre_CD (Addgene #66030), DVA (Addgene #66056), DVA_AB (Addgene #66039), DVA_AE (Addgene #66040), DVA_AF (Addgene #66041), DVA_AG (Addgene #66042), DVA_AH (Addgene #66043), DVA_BC (Addgene #66044), DVA_CD (Addgene #66045), DVA_DE (Addgene #66046), DVA_DF (Addgene #66047), DVA_DG (Addgene #66048), DVA_DH (Addgene #66049), DVA_EB (Addgene #66050), DVA_EF (Addgene #66051), DVA_EG (Addgene #66052), DVA_EH (Addgene #66053), DVA_FB (Addgene #66054), DVA_GB (Addgene #66055), DVK (Addgene #66072), DVK_AE (Addgene #66067), DVK_AF (Addgene #66068), DVK_EF (Addgene #66069), DVK_FG (Addgene #66070), DVK_GH (Addgene #66071), E0030 (YFP)_CD (Addgene #66031), E0040m (GFP)_CD (Addgene #66032), E1010m (RFP)_CD (Addgene #66033), eBFP2 (eBFP2)_CD (Addgene #66034), I13453 (pBAD)_AB (Addgene #66016), I13453 (pBAD)_EB (Addgene #66017), I13453 (pBAD)_FB (Addgene #66018), I13453 (pBAD)_GB (Addgene #66019), J23100_AB (Addgene #65980), J23100_EB (Addgene #65981), J23100_FB (Addgene #65982), J23100_GB (Addgene #65983), J23102_AB (Addgene #65984), J23102_EB (Addgene #65985), J23102_FB (Addgene #65986), J23102_GB (Addgene #65987), J23103_AB (Addgene #65988), J23103_EB (Addgene #65989), J23103_FB (Addgene #65990), J23103_GB (Addgene #65991), J23106_AB (Addgene #65992), J23106_EB (Addgene #65993), J23106_FB (Addgene #65994), J23106_GB (Addgene #65995), J23107_AB (Addgene #65996), J23107_EB (Addgene #65997), J23107_FB (Addgene #65998), J23107_GB (Addgene #65999), J23116_AB (Addgene #66000), J23116_EB (Addgene #66001), J23116_FB (Addgene #66002), J23116_GB (Addgene #66003), pJ02B2Gm:Rm_AF (Addgene #66061), pJ02B2Gm_AE (Addgene #66057), pJ02B2Gm_AE (Addgene #66063), pJ02B2Gm_EF (Addgene #66058), pJ02B2Gm_EF (Addgene #66064), pJ02B2Rm:Gm_AF (Addgene #66062), pJ02B2Rm_AE (Addgene #66059), pJ02B2Rm_AE (Addgene #66065), pJ02B2Rm_EF (Addgene #66060), pJ02B2Rm_EF (Addgene #66066), R0010 (pLacI)_AB (Addgene #66004), R0010 (pLacI)_EB (Addgene #66005), R0010 (pLacI)_FB (Addgene #66006), R0010 (pLacI)_GB (Addgene #66007), R0040 (pTet)_AB (Addgene #66008), R0040 (pTet)_EB (Addgene #66009), R0040 (pTet)_FB (Addgene #66010), R0040 (pTet)_GB (Addgene #66011), R0063 (pLuxR-pR)_AB (Addgene #66012), R0063 (pLuxR-pR)_EB (Addgene #66013), R0063 (pLuxR-pR)_FB (Addgene #66014), R0063 (pLuxR-pR)_GB (Addgene #66015)
Proper citation: RRID:Addgene_1000000059 Copy
https://www.addgene.org/pooled-library/zhang-human-sam-v1/
Vector Backbone Description: Vector Types:CRISPR, Lentiviral, Mammalian Expression
Comments: Human SAM library (Addgene #61597), lenti dCAS-VP64_Blast (Addgene #61425), lentiMPH v2 (Addgene #89308)
Proper citation: RRID:Addgene_1000000057 Copy
https://www.addgene.org/kits/pertz-cpfret-biosensors/
Vector Backbone Description: Vector Types:Bacterial Expression, Insect Expression, Mammalian Expression
Comments: EKAR2G_design1_mTFP_105_Venus_157 (Addgene #39819), EKAR2G_design1_mTFP_105_Venus_173 (Addgene #39820), EKAR2G_design1_mTFP_105_Venus_195 (Addgene #39821), EKAR2G_design1_mTFP_105_Venus_229 (Addgene #39822), EKAR2G_design1_mTFP_105_Venus_wt (Addgene #39818), EKAR2G_design1_mTFP_159_Venus_157 (Addgene #39824), EKAR2G_design1_mTFP_159_Venus_173 (Addgene #39825), EKAR2G_design1_mTFP_159_Venus_195 (Addgene #39826), EKAR2G_design1_mTFP_159_Venus_229 (Addgene #39827), EKAR2G_design1_mTFP_159_Venus_wt (Addgene #39823), EKAR2G_design1_mTFP_175_Venus_157 (Addgene #39829), EKAR2G_design1_mTFP_175_Venus_173 (Addgene #39830), EKAR2G_design1_mTFP_175_Venus_195 (Addgene #39831), EKAR2G_design1_mTFP_175_Venus_229 (Addgene #39832), EKAR2G_design1_mTFP_175_Venus_wt (Addgene #39828), EKAR2G_design1_mTFP_227_Venus_157 (Addgene #39834), EKAR2G_design1_mTFP_227_Venus_173 (Addgene #39835), EKAR2G_design1_mTFP_227_Venus_195 (Addgene #39836), EKAR2G_design1_mTFP_227_Venus_229 (Addgene #39837), EKAR2G_design1_mTFP_227_Venus_wt (Addgene #39833), EKAR2G_design1_mTFP_wt_Venus_157 (Addgene #39814), EKAR2G_design1_mTFP_wt_Venus_173 (Addgene #39815), EKAR2G_design1_mTFP_wt_Venus_195 (Addgene #39816), EKAR2G_design1_mTFP_wt_Venus_229 (Addgene #39817), EKAR2G_design1_mTFP_wt_Venus_wt (Addgene #39813), EKAR2G_design2_mTFP_105_Venus_157 (Addgene #40000), EKAR2G_design2_mTFP_105_Venus_173 (Addgene #40001), EKAR2G_design2_mTFP_105_Venus_195 (Addgene #40002), EKAR2G_design2_mTFP_105_Venus_229 (Addgene #40003), EKAR2G_design2_mTFP_105_Venus_wt (Addgene #39999), EKAR2G_design2_mTFP_159_Venus_157 (Addgene #40005), EKAR2G_design2_mTFP_159_Venus_173 (Addgene #40006), EKAR2G_design2_mTFP_159_Venus_195 (Addgene #40007), EKAR2G_design2_mTFP_159_Venus_229 (Addgene #40008), EKAR2G_design2_mTFP_159_Venus_wt (Addgene #40004), EKAR2G_design2_mTFP_175_Venus_157 (Addgene #40010), EKAR2G_design2_mTFP_175_Venus_173 (Addgene #40011), EKAR2G_design2_mTFP_175_Venus_195 (Addgene #40012), EKAR2G_design2_mTFP_175_Venus_229 (Addgene #40013), EKAR2G_design2_mTFP_175_Venus_wt (Addgene #40009), EKAR2G_design2_mTFP_227_Venus_157 (Addgene #40015), EKAR2G_design2_mTFP_227_Venus_173 (Addgene #40016), EKAR2G_design2_mTFP_227_Venus_195 (Addgene #40017), EKAR2G_design2_mTFP_227_Venus_229 (Addgene #40018), EKAR2G_design2_mTFP_227_Venus_wt (Addgene #40014), EKAR2G_design2_mTFP_wt_Venus_157 (Addgene #39995), EKAR2G_design2_mTFP_wt_Venus_173 (Addgene #39996), EKAR2G_design2_mTFP_wt_Venus_195 (Addgene #39997), EKAR2G_design2_mTFP_wt_Venus_229 (Addgene #39998), EKAR2G_design2_mTFP_wt_Venus_wt (Addgene #39994)
Proper citation: RRID:Addgene_1000000021 Copy
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