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Species: Other
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725297.1
Proper citation: RRID:Addgene_145699 Copy
Species: Other
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725297.1
Proper citation: RRID:Addgene_145747 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725307.1
Proper citation: RRID:Addgene_145754 Copy
Species: Other
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724392.1
Proper citation: RRID:Addgene_145751 Copy
Species: Other
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724395.1
Proper citation: RRID:Addgene_145701 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725305.1
Proper citation: RRID:Addgene_145702 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725302.1
Proper citation: RRID:Addgene_145709 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725303.1
Proper citation: RRID:Addgene_145703 Copy
Species: Drosophila melanogaster
Genetic Insert: DmLSm1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17923697
Proper citation: RRID:Addgene_145880 Copy
Species: Drosophila melanogaster
Genetic Insert: Dmp68
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: ORF extracted from NCBI:NM_169118.1. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145955 Copy
Species: Homo sapiens
Genetic Insert: HsRck
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145972 Copy
Species: E. coli Tn7
Genetic Insert: TnsC(A225V)
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pGD108; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9055068
Proper citation: RRID:Addgene_17257 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854235
Proper citation: RRID:Addgene_172604 Copy
Genetic Insert: TetON-P2A-TetOFF-T2A-bsd
Vector Backbone Description: Backbone Size:7821; Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31268602
Comments: The plasmid can be used in conjunction with pLenti-Tet (Addgene #138086) that has the ORF for the gene of interest of which expression can then be induced with Tetracycline or Doxycyline in Mammalian cells.
Proper citation: RRID:Addgene_138085 Copy
Vector Backbone Description: Vector Backbone:pH6HTN; Vector Types:Bacterial Expression, Other, Cell Free Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_175298 Copy
Genetic Insert: Adenovirus 5 genomic region 25043-35938
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Adenoviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35098167
Proper citation: RRID:Addgene_175747 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mitochondrial FAD-linked sulfhydryl oxidase
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176405 Copy
Species: Other
Genetic Insert: FAD-linked sulfhydryl oxidase ERV2 from Fol
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176404 Copy
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2194165
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.
Proper citation: RRID:Addgene_1765 Copy
Species: Other
Genetic Insert: GFP-GUS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8000; Vector Backbone:pB7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28784835
Proper citation: RRID:Addgene_167122 Copy
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