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Species: Homo sapiens
Genetic Insert: HTR3D
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_161626 Copy
Species: Mus musculus
Genetic Insert: Kir2.1-2A-GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28902833
Proper citation: RRID:Addgene_161574 Copy
Species: Mus musculus
Genetic Insert: Kir2.1-2A-GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28902833
Proper citation: RRID:Addgene_161577 Copy
Species: Homo sapiens
Genetic Insert: hM4Di-mCherry
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28902833
Proper citation: RRID:Addgene_161579 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Gentamicin
Proper citation: RRID:Addgene_161881 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161882 Copy
Species: Synthetic
Genetic Insert: ExRai-AKAR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5513; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989297
Proper citation: RRID:Addgene_161753 Copy
Species: Other
Genetic Insert: human codon optimized SpCas9
Vector Backbone Description: Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25607456
Proper citation: RRID:Addgene_161876 Copy
Species: Synthetic
Genetic Insert: ExRai-AKAR2(T/A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5513; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989297
Proper citation: RRID:Addgene_161754 Copy
Species: Geobacillus stearothermophilus
Genetic Insert: Modified DNA Polymerase I Large Fragment (Br512)
Vector Backbone Description: Backbone Marker:In-house made; Backbone Size:5187; Vector Backbone:pKAR2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.10.02.20203356v1 for medRxiv preprint.
Proper citation: RRID:Addgene_161875 Copy
Species: RsSHC014 bat coronavirus
Genetic Insert: Receptor-binding domain (RBD) of spike protein S
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33597251
Proper citation: RRID:Addgene_161826 Copy
Species: Synthetic
Genetic Insert: ABE8e base editor
Vector Backbone Description: Backbone Marker:Atum; Backbone Size:2250; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33462442
Proper citation: RRID:Addgene_161788 Copy
Species: Synthetic
Genetic Insert: Luciferase-T2A-HSV-TK
Vector Backbone Description: Backbone Marker:Systems Biosciences; Backbone Size:8000; Vector Backbone:pLenti-Luc-2A-Ctx; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31398338
Proper citation: RRID:Addgene_161789 Copy
Species: Rs4084 bat coronavirus
Genetic Insert: Receptor-binding domain (RBD) of spike protein S
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33597251
Proper citation: RRID:Addgene_161825 Copy
Species: Homo sapiens
Genetic Insert: bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_161816 Copy
Species: Homo sapiens
Genetic Insert: bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_161815 Copy
Species: Homo sapiens
Genetic Insert: Growth factor independent 1B
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGCCACGCTCCTTCCTGG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG CTTGAGATTGTGTTGACTCTC-3'.
Proper citation: RRID:Addgene_16169 Copy
Species: Homo sapiens
Genetic Insert: Ubiquilin 4
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGCGGAGCCGAGCGG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GGAGAGCTGGGAGCCCAG-3'.
Proper citation: RRID:Addgene_16170 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:6625; Vector Backbone:pSBbi-RP (Addgene Plasmid #60513); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36619904
Comments: The SARS-CoV-2 RBD insert was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]).
This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate stable mammalian cells for the production of SARS-CoV-2 RBD protein. This protein can be recovered from the cell culture medium by His-tag purification.
The recombinant RBD is suitable for COVID-19 serology testing and monitoring of humoral responses in vaccinated population.
Proper citation: RRID:Addgene_161793 Copy
Species: Homo sapiens
Genetic Insert: Quaking
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGTCGGGGAAATGGAAAC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTTGCCGGTGGCGGCTCG-3'.
Proper citation: RRID:Addgene_16183 Copy
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