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Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Chunbo Lou; Backbone Size:6468; Vector Backbone:pTHS; Vector Types:CRISPR, Integrative vector for use in Streptomyces; Bacterial Resistance:Hygromycin
Defining Citation: PMID:37801665
Proper citation: RRID:Addgene_210387 Copy
Genetic Insert: this strain is a P2 lysogen derived from E. coli EMG C5545 strain
Vector Backbone Description: Vector Backbone:This is a strain.; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:36731126
Proper citation: RRID:Addgene_197132 Copy
Genetic Insert: GFP(47TAG)
Vector Backbone Description: Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28276676
Comments: Plasmid was originally reported by the Peter Schultz lab in Wang, Schultz et al. PLoS One 2010, doi: 10.1371/journal.pone.0009354. Please see associated publication Ganapathy, Seeliger et al. for full details regarding any additional cloning that was performed.
Proper citation: RRID:Addgene_193251 Copy
Genetic Insert: none, cloning vector for introduction of targeting sequences
Vector Backbone Description: Vector Backbone:pCHERRY3 : pAL5000/colE1 origins; Vector Types:CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:38319218
Proper citation: RRID:Addgene_221387 Copy
Vector Backbone Description: Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39302127
Comments: Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B.
Proper citation: RRID:Addgene_222351 Copy
Species: Neonothopanus nambi
Genetic Insert: pGAP - nnH3H_v2 - tAOX
Vector Backbone Description: Backbone Size:4113; Vector Backbone:Level 1-like; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:38253843
Proper citation: RRID:Addgene_219752 Copy
Species: Aspergillus nidulans
Genetic Insert: pGAP - npgA - tAOX
Vector Backbone Description: Backbone Size:3503; Vector Backbone:Level 1-like; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:38253843
Proper citation: RRID:Addgene_219750 Copy
Vector Backbone Description: Backbone Marker:Addgene 25012; Backbone Size:6000; Vector Backbone:pKW-08; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:27114277
Proper citation: RRID:Addgene_249819 Copy
Species: Mycobacterium tuberculosis
Genetic Insert: Mycobacterium tuberculosis topoisomerase I
Vector Backbone Description: Backbone Marker:Addgene 25012; Backbone Size:6000; Vector Backbone:pKW-08; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:27114277
Proper citation: RRID:Addgene_249818 Copy
Vector Backbone Description: Backbone Size:5888; Vector Backbone:pKEK2201; Vector Types:Sucrose counter-selection allelic exchange vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39566662
Proper citation: RRID:Addgene_252057 Copy
Species: Mycobacterium smegmatis MC2-155
Genetic Insert: 3' mmpL3(MSMEG_0250)-ALFA
Vector Backbone Description: Vector Backbone:pAJF229; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:40576343
Proper citation: RRID:Addgene_252905 Copy
Species: Synthetic
Genetic Insert: TIMER
Vector Backbone Description: Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:42153669
Comments: Timer protein, a derivative of DsRed, undergoes a spontaneous and irreversible green-to-red emission shift as it matures over time. Newly synthesized Timer molecules emit green fluorescence, which gradually shifts to red over time. Bacteria can be identified as actively replicating (green), intermediate (orange), or non-replicating (red) based on their green-to-red fluorescence ratio.
Proper citation: RRID:Addgene_258482 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225859 Copy
Species: Synthetic
Genetic Insert: CRISPRt transposon with gRNA expression cassette (mScar-T03 guide)
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225844 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (LZ1 guide) and synthetic promoter H
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225861 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (no guide) and synthetic promoter H
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225860 Copy
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