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pJN105-Hygro-GFP
 
Resource Report
Resource Website 		RRID:Addgene_41883 Ptac::GFP-mut-2 A. victoria Hygromycin PMID:15271914 The pBBR1MCS-5 derivative of the Bordetella bronchiseptica BHR plasmid has a pBBR/BHR replicon that is maintained in L. pneumophila and is compatible with IncP, IncW, IncQ, and IncRi incompatibility groups. Plasmid pJN105 is a derivative of pBBR1MCS-5 with araC and PBAD regions before the polylinker. PCR primers with BglII sites at their 5′ ends were used to amplify the region outside the gentamicin cassette in pJN105. The PCR fragment was digested with BglII, blunted with the Klenow fragment, and ligated to the blunted DdeI-DdeI hygromycin resistance fragment from pMV206.hygro to form pJN105-hygro. The Ptac-GFP fragment of pGS-GFP04 was amplified by PCR by using primers with NheI sites at their 5′ ends. After digestion with NheI, this fragment was ligated with NheI-digested pJN105-hygro to form pJN105-hygro-GFP. Backbone Size:6050; Vector Backbone:pJN105; Vector Types:Bacterial Expression, Bacterial broad host range vector; Bacterial Resistance:Hygromycin GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser72Ala 2026-09-01 09:54:32 0
pMyNT
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_42191 Hygromycin PMID:33006405 Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München) Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-01 09:54:36 3
pBabe-Hygro-RAS
 
Resource Report
Resource Website 		RRID:Addgene_242639 HRASG12V Homo sapiens Hygromycin PMID:40737085 Vector Backbone:pBabe; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin G12V 2026-09-01 09:47:52 0
pCap-101-Hgy
 
Resource Report
Resource Website 		RRID:Addgene_227508 Hygromycin PMID:39666857 Backbone Size:9662; Vector Backbone:pBAC2015, pQS-gusA, and pUC19; Vector Types:; Bacterial Resistance:Hygromycin 2026-09-01 09:43:39 0
pRel-Hyg
 
Resource Report
Resource Website 		RRID:Addgene_227582 Cas9 Hygromycin PMID:39666857 Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Hygromycin 2026-09-01 09:43:40 0
pSF511
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_222719 gRNA1(gsdA) Other Hygromycin Backbone Marker:Michael Sauer (Sarkari et al. 2017) Addgene #90278; Vector Backbone:pMST665_BB3_L 23_gRNAempty_cas9_BbsI; Vector Types:Bacterial Expression, CRISPR, A. niger; Bacterial Resistance:Hygromycin 2026-09-01 09:42:23 1
pRK2-A281virF
 
Resource Report
Resource Website 		RRID:Addgene_243807 virF-like gene Agrobacterium tumefaciens A281 Hygromycin Vector Backbone:pRK2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin 2026-09-01 09:48:03 0
pTA-noI
 
Resource Report
Resource Website 		RRID:Addgene_249819 Hygromycin PMID:27114277 Backbone Marker:Addgene 25012; Backbone Size:6000; Vector Backbone:pKW-08; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-01 09:49:37 0
pTA-M+
 
Resource Report
Resource Website 		RRID:Addgene_249818 Mycobacterium tuberculosis topoisomerase I Mycobacterium tuberculosis Hygromycin PMID:27114277 Backbone Marker:Addgene 25012; Backbone Size:6000; Vector Backbone:pKW-08; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-01 09:49:37 0
pVAM07
 
Resource Report
Resource Website 		RRID:Addgene_252057 Hygromycin PMID:39566662 Backbone Size:5888; Vector Backbone:pKEK2201; Vector Types:Sucrose counter-selection allelic exchange vector; Bacterial Resistance:Hygromycin 2026-09-01 09:50:09 0
pAJF1126
 
Resource Report
Resource Website 		RRID:Addgene_252905 3' mmpL3(MSMEG_0250)-ALFA Mycobacterium smegmatis MC2-155 Hygromycin PMID:40576343 Vector Backbone:pAJF229; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-01 09:50:17 0
Mtb-Timer
 
Resource Report
Resource Website 		RRID:Addgene_258482 TIMER Synthetic Hygromycin PMID:42153669 Timer protein, a derivative of DsRed, undergoes a spontaneous and irreversible green-to-red emission shift as it matures over time. Newly synthesized Timer molecules emit green fluorescence, which gradually shifts to red over time. Bacteria can be identified as actively replicating (green), intermediate (orange), or non-replicating (red) based on their green-to-red fluorescence ratio. Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin codon-optimized for Mycobacterium tuberculosis 2026-09-01 09:51:20 0
pJMP15011_pCRISPRtOE-noP-hyg
 
Resource Report
Resource Website 		RRID:Addgene_225859 CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter Synthetic Hygromycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin 2026-09-01 09:43:12 0
pJMP10751_pCRISPRt-T-hyg-T03
 
Resource Report
Resource Website 		RRID:Addgene_225844 CRISPRt transposon with gRNA expression cassette (mScar-T03 guide) Synthetic Hygromycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin 2026-09-01 09:43:11 0
pJMP15021_pCRISPRtOE-PH-hyg-LZ1
 
Resource Report
Resource Website 		RRID:Addgene_225861 CRISPRtOE transposon with gRNA expression cassette (LZ1 guide) and synthetic promoter H Synthetic Hygromycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin 2026-09-01 09:43:12 0
pJMP15014_pCRISPRtOE-PH-hyg
 
Resource Report
Resource Website 		RRID:Addgene_225860 CRISPRtOE transposon with gRNA expression cassette (no guide) and synthetic promoter H Synthetic Hygromycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Hygromycin 2026-09-01 09:43:12 0

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The Stimulating Peripheral Activity to Relieve Conditions (SPARC) effort is the result of National Institutes of Health’s (NIH) drive to map out the neural circuitry responsible for visceral control in higher vertebrates. The SPARC Anatomy Working Group (SAWG) is responsible for the integrity of anatomical knowledge in SPARC

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