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Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pDAI-SceI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25172289
Comments: Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function
Proper citation: RRID:Addgene_113635 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649424 , Alternative ID = pGT403 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114164 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649422, Alternative ID = pGT401 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114162 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649425 , Alternative ID = pGT406 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114167 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649426, Alternative ID = pGT405 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114166 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL4218 (mtlB) containing all of RL4219
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83787 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL3424 (dctA)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83786 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream pRL90085 containing all of pRL90087
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83788 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream pRL120556 containing all of pRL120555
Vector Backbone Description: Backbone Marker:16260; Backbone Size:17558; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83781 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: upstream region of RL4655 (intA)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83780 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL4393 (fdsG) containing all of RL4394
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83783 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL0489 (frcB) containing all of RL0488 (frcR)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83792 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL0102 (gabT) containing all of RL0103
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83791 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: para-cyanophenylalanine Mj synthetase
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:19492814
Proper citation: RRID:Addgene_85494 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: 3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24611875
Proper citation: RRID:Addgene_85498 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20839808
Comments: This synthetase is permissive and can incorporate a variety of amino acids structurally analogous to IBBN. For a more complete functional and structural analysis of this synthetase, also see Cooley et al. Chembiochem. 2014, 15(12): 1810–1819.
Proper citation: RRID:Addgene_85500 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20082521
Proper citation: RRID:Addgene_85484 Copy
Species: S. meliloti
Genetic Insert: exoY
Vector Backbone Description: Backbone Marker:Kay Jones; Vector Backbone:pstb-LAFR5; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22685282
Proper citation: RRID:Addgene_86170 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111252 Copy
Species: Synthetic
Genetic Insert: TorA signal peptide
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111253 Copy
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