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URL: https://n2t.net/bdsc:81891
Proper Citation: RRID:BDSC_81891
Description: Drosophila melanogaster with name w[*]; P{w[+mC]=UAS-mCherry.NLS}2, PBac{y[+mDint2] w[+mC]=U6-GFP.gRNA.ACDG}VK00037/CyO, P{Wee-P.ph0}2; P{w[+mC]=UAS-mCD8::GFP.L}LL6, P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1] from BDSC.
Species: Drosophila melanogaster
Notes: Expresses sgRNA targeting GFP under the control of U6 promoter. Ubiquitous expression of mCD8-GFP and RFP. When crossed to a Cas9-expressing stock, sgRNA-induced mutations result in a loss of GFP signal in Cas9-expressing cells (negative tester NT2). Donor: Chun Han, Cornell University
Affected Gene: Avic\GFP, snRNA:U6:96Aa, alphaTub84B, GAL4, UAS, Disc\RFP, unknown (enhancer trap), Tb, w
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No rating or validation information has been found for w[*]; P{w[+mC]=UAS-mCherry.NLS}2, PBac{y[+mDint2] w[+mC]=U6-GFP.gRNA.ACDG}VK00037/CyO, P{Wee-P.ph0}2; P{w[+mC]=UAS-mCD8::GFP.L}LL6, P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1].
No alerts have been found for w[*]; P{w[+mC]=UAS-mCherry.NLS}2, PBac{y[+mDint2] w[+mC]=U6-GFP.gRNA.ACDG}VK00037/CyO, P{Wee-P.ph0}2; P{w[+mC]=UAS-mCD8::GFP.L}LL6, P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1].
Source: Integrated Animals
Source Database: Bloomington Drosophila Stock Center (BDSC)