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URL: http://www.addgene.org/70694
Proper Citation: RRID:Addgene_70694
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17639350
Vector Backbone Description: Backbone Marker:Wolk Lab; Backbone Size:7432; Vector Backbone:pRL25; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Kanamycin
Comments: MCS: BamHI-AscI-SrfI-XhoI(PspXI)-BsiWI-AatII(ZraI)-AvrII-StuI-NsiI-SacII-ApaI-ClaI-EcoRI-SacI(EcoICRI)-KpnI(Acc65I)-SalI-SbfI-NotI Medium copy number in E. coli. The literature (e.g., Yang et al., Res. in Microbiol. [Inst. Pasteur] 164, 127-135, 2013) says that there is substantial variability in the copy number of pDU1-based plasmids in Anabaena PCC 7120 dependent on the insert in the plasmid.
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Source: Addgene