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URL: http://www.addgene.org/70688
Proper Citation: RRID:Addgene_70688
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:8412673
Vector Backbone Description: Vector Backbone:see comments; Vector Types:; Bacterial Resistance:Chloramphenicol
Comments: MCS: EcoRV-BglII-XhoI-BssHII-SpeI-BsiWI-AvrII-AflII-BstBI and potentially other sites sacB was derived from pUM24 (Ried, J. L., and A. Colimer. Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984: Gene 32: 481-485) and a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985: Gene 37: 299-304). The erythromycin-resistance gene came from Horinouchi, S., and Weisblum, B. (J. Bacteriol. 150: 804-814, 1982).
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Source: Addgene