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URL: http://www.addgene.org/70686
Proper Citation: RRID:Addgene_70686
Insert Name: Transposon Tn5-1058
Bacterial Resistance: Kanamycin and Streptomycin
Defining Citation: PMID:11607193
Vector Backbone Description: Vector Backbone:Transposon Tn5, variously modified; Vector Types:; Bacterial Resistance:Kanamycin and Streptomycin
Comments: Tn5 derivative Tn5-1058 (in plasmid pRL1058) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 is this series of plasmids), providing large numbers of transposon mutants. The XbaI site near the L end of Tn5 is the sole unmethylated XbaI in the construct, allowing the placement of luciferases (e.g., Vibrio fischeri luxAB as in pRL1063a). An oriT for conjugal transfer is positioned between the L and R ends of the Tn.
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Source: Addgene