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URL: http://www.addgene.org/70188
Proper Citation: RRID:Addgene_70188
Insert Name: amyEopt
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:27333161
Vector Backbone Description: Backbone Marker:Fagan and Fairweather 2009; Backbone Size:7216; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note: the depositing lab has determined that a particular IS element present in E. coli strains such as DH5alpha and its derivatives has a high propensity to insert into this plasmid. The depositing lab recommends isolating the plasmid DNA and transforming into a strain similar to MC1061. Publication describing the backbone vector pRPF185 as well as the sequencing primers: Clostridium difficile has two parallel and essential Sec secretion systems. Fagan RP, Fairweather NF. J Biol Chem. 2011 Aug 5;286(31):27483-93. doi: 10.1074/jbc.M111.263889. Epub 2011 Jun 9. PMID: 21659510
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Source: Addgene
