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URL: http://www.addgene.org/69946
Proper Citation: RRID:Addgene_69946
Bacterial Resistance: Kanamycin
Vector Backbone Description: Vector Backbone:pMY200; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Comments: PCR was used to amplify a fragment containing the left T-DNA border from PBht2. Two primers were designed as follows: TRDNAS: CAAAgTTggCgTATAACATAgT, downstream of SacII site in pBht2. TLDNAAPmeI: ATTATAgTTTAAACATTCggCgTTAATTCAgTACA, upstream of T-border 5’ end contains PmeI site in pBht2. 2. Digested PCR product with PmeI and SacII, gel extraction followed by recovery of ~400 bp fragment, and pBht2 vector was digested with PmeI and SacII, gel extracted, followed by recovery of ~6 kb fragment. CIP dephosphatase treatment, ligated with PCR product, electroporate into E. coli.
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Source: Addgene
