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URL: http://www.addgene.org/131465
Proper Citation: RRID:Addgene_131465
Insert Name: Codon optimized SpCas9-NG
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31501500
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of SpCas9-NG shown in previous study (Nishimasu H et al., Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science, 2018).
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Source: Addgene
